iTRAQ-based quantitative proteomic analysis of cerebrospinal fluid reveals NELL2 as a potential diagnostic biomarker of tuberculous meningitis

  • Authors:
    • Yongtao Yang
    • Jun Mu
    • Guanghui Chen
    • Yuan Zhan
    • Jiaju Zhong
    • Youdong Wei
    • Ke Cheng
    • Bin Qin
    • Hongmin You
    • Peng Xie
  • View Affiliations

  • Published online on: March 11, 2015     https://doi.org/10.3892/ijmm.2015.2131
  • Pages: 1323-1332
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Abstract

Tuberculous meningitis (TBM) is a serious complication of tuberculosis that affects the central nervous system. As TBM may result in permanent sequelae and death, rapid, accurate diagnostic tests using novel biomarkers are required for the early diagnosis and treatment of TBM. A quantitative proteomic study was therefore performed to identify differential proteins in the cerebrospinal fluid (CSF) obtained from TBM patients (n=12) and healthy controls (n=12). CSF samples were labelled with iTRAQ™ and analyzed by LC‑MS/MS. Gene ontology and Pathway analysis were conducted using DAVID bioinformatics resources. Neural epidermal growth factor‑like like 2 (NELL2) with the largest fold‑change value was selected for validation by western blotting. Proteomic phenotyping revealed over‑representation in two inflammation‑associated processes, complement and coagulation cascades as well as cell adhesion molecules. Western blotting showed a significant decrease in NELL2 levels in TBM subjects compared to healthy controls. The AUC analysis revealed NELL2 was able to distinguish TBM subjects from healthy controls with 83.3% sensitivity and 75% specificity. In conclusion, the results showed that CSF NELL2 is a potential diagnostic biomarker for TBM. Further evaluation of these findings in larger studies including anti‑tuberculosis medicated and unmedicated patient cohorts with other intracranial infectious diseases is required for clinical translation.
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May-2015
Volume 35 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yang Y, Mu J, Chen G, Zhan Y, Zhong J, Wei Y, Cheng K, Qin B, You H, Xie P, Xie P, et al: iTRAQ-based quantitative proteomic analysis of cerebrospinal fluid reveals NELL2 as a potential diagnostic biomarker of tuberculous meningitis. Int J Mol Med 35: 1323-1332, 2015
APA
Yang, Y., Mu, J., Chen, G., Zhan, Y., Zhong, J., Wei, Y. ... Xie, P. (2015). iTRAQ-based quantitative proteomic analysis of cerebrospinal fluid reveals NELL2 as a potential diagnostic biomarker of tuberculous meningitis. International Journal of Molecular Medicine, 35, 1323-1332. https://doi.org/10.3892/ijmm.2015.2131
MLA
Yang, Y., Mu, J., Chen, G., Zhan, Y., Zhong, J., Wei, Y., Cheng, K., Qin, B., You, H., Xie, P."iTRAQ-based quantitative proteomic analysis of cerebrospinal fluid reveals NELL2 as a potential diagnostic biomarker of tuberculous meningitis". International Journal of Molecular Medicine 35.5 (2015): 1323-1332.
Chicago
Yang, Y., Mu, J., Chen, G., Zhan, Y., Zhong, J., Wei, Y., Cheng, K., Qin, B., You, H., Xie, P."iTRAQ-based quantitative proteomic analysis of cerebrospinal fluid reveals NELL2 as a potential diagnostic biomarker of tuberculous meningitis". International Journal of Molecular Medicine 35, no. 5 (2015): 1323-1332. https://doi.org/10.3892/ijmm.2015.2131