Erythronium japonicum attenuates histopathological lung abnormalities in a mouse model of ovalbumin-induced asthma

Seo,Ji-Hye; Bang,Mi-Ae; Kim,Gyeyeop; Cho,Seung Sik; Park,Dae-Hun

doi:10.3892/ijmm.2016.2541

Erythronium japonicum attenuates histopathological lung abnormalities in a mouse model of ovalbumin-induced asthma

Authors:
- Ji-Hye Seo
- Mi-Ae Bang
- Gyeyeop Kim
- Seung Sik Cho
- Dae-Hun Park
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Affiliations: Department of Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 58245, Republic of Korea, R&D Team, Jeonnam Bioindustry Foundation, Food Research Institute (JBF-FRI), Naju, Jeonnam 58275, Republic of Korea, Department of Physical Therapy, Dongshin University, Naju, Jeonnam 58245, Republic of Korea, College of Pharmacy, Mokpo National University, Mokpo, Jeonnam 588554, Republic of Korea
Published online on: March 28, 2016 https://doi.org/10.3892/ijmm.2016.2541
Pages: 1221-1228
Copyright: © Seo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Asthma is a chronic lung condition that can induce mucus hypersecretion and pulmonary obstruction and may even cause death, particularly in children and older individuals. Erythronium japonicum (E. japonicum) is a traditional herb used in Korea and East Asian countries that has been found to exert free radical scavenging activity and anti-proliferative effects in human colorectal carcinoma cells. In the present study, we evaluated the anti-asthmatic effects of an extract of E. japonicum in a mouse model of ovalbumin (OVA)‑induced asthma. Female BALB/c mice were sensitized with an intraperitoneal injection of OVA and aluminum hydroxide hydrate on days 1 and 8 and then received the following treatments on days 21 to 25: i) control (no treatment), ii) sterilized tap water (given orally), iii) 1 mg/kg/day dexamethasone (administered orally), iv) 60 mg/kg/day E. japonicum extract, and v) 600 mg/kg/day E. japonicum extract. On the same days, all the mice except those in the control group were challenged 1 h later with nebulized 5% OVA for 30 min. We found that treatment with E. japonicum extract suppressed the OVA-induced increase in the number of white blood cells and decreased the IgE level in the bronchoalveolar lavage fluid samples obtained from the mice. Histopathological analysis of the lung tissues revealed that E. japonicum attenuated the asthma-related morphological changes in the mouse lung tissue, including the increased secretion of mucus in the bronchioles, eosinophil infiltration around the bronchioles and vessels, and goblet cell and epithelial cell hyperplasia. Immunohistochemical analysis revealed that treatment with E. japonicum extract suppressed the OVA-induced proliferation of T helper cells (CD4+) and B cells (CD19+) in the mouse lung tissue. Furthermore, treatment with E. japonicum extract modulated the expression of both T helper 2 cell-related factors [GATA binding protein 3 (GATA-3), tumor necrosis factor-α (TNF‑α), interleukin (IL)-4, IL-5, IL-6 and IL-13], as well as that of T helper 1 cell-related factors [(interferon-γ (IFN-γ), IL-12p35 and IL-12p40]. These findings suggest that E. japonicum may potentially be used as an anti-asthmatic treatment.

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1	World Health Organization: Asthma Fact Sheet No307. November. 2013
2	United States Environmental Protection Agency (EPA): Asthma Facts EPA-402-F-04-019. March. 2013
3	Slejko JF, Ghushchyan VH, Sucher B, Globe DR, Lin SL, Globe G and Sullivan PW: Asthma control in the United States, 2008–2010: indicators of poor asthma control. J Allergy Clin Immunol. 133:1579–1587. 2014. View Article : Google Scholar
4	Kay AB: Allergy and allergic diseases. First of two parts. N Engl J Med. 344:30–37. 2001. View Article : Google Scholar : PubMed/NCBI
5	National Asthma Education and Prevention Program: Expert Panel Report: Guidelines for the Diagnosis and Management of Asthma Update on Selected Topics - 2002. J Allergy Clin Immunol. 110(Suppl 5): S141–S219. 2002.
6	Mattes J, Yang M, Mahalingam S, Kuehr J, Webb DC, Simson L, Hogan SP, Koskinen A, McKenzie AN, Dent LA, et al: Intrinsic defect in T cell production of interleukin (IL)-13 in the absence of both IL-5 and eotaxin precludes the development of eosinophilia and airways hyperreactivity in experimental asthma. J Exp Med. 195:1433–1444. 2002. View Article : Google Scholar : PubMed/NCBI
7	Manetti R, Parronchi P, Giudizi MG, Piccinni MP, Maggi E, Trinchieri G and Romagnani S: Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells. J Exp Med. 177:1199–1204. 1993. View Article : Google Scholar : PubMed/NCBI
8	Szabo SJ, Kim ST, Costa GL, Zhang X, Fathman CG and Glimcher LH: A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell. 100:655–669. 2000. View Article : Google Scholar : PubMed/NCBI
9	Zhu J, Jankovic D, Oler AJ, Wei G, Sharma S, Hu G, Guo L, Yagi R, Yamane H, Punkosdy G, et al: The transcription factor T-bet is induced by multiple pathways and prevents an endogenous Th2 cell program during Th1 cell responses. Immunity. 37:660–673. 2012. View Article : Google Scholar : PubMed/NCBI
10	Brightling CE, Symon FA, Birring SS, Bradding P, Pavord ID and Wardlaw AJ: TH2 cytokine expression in bronchoalveolar lavage fluid T lymphocytes and bronchial submucosa is a feature of asthma and eosinophilic bronchitis. J Allergy Clin Immunol. 110:899–905. 2002. View Article : Google Scholar : PubMed/NCBI
11	Mosmann TR and Coffman RL: TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties. Annu Rev Immunol. 7:145–173. 1989. View Article : Google Scholar : PubMed/NCBI
12	Yagi R, Zhu J and Paul WE: An updated view on transcription factor GATA3-mediated regulation of Th1 and Th2 cell differentiation. Int Immunol. 23:415–420. 2011. View Article : Google Scholar : PubMed/NCBI
13	Barnes PJ: Immunology of asthma and chronic obstructive pulmonary disease. Nat Rev Immunol. 8:183–192. 2008. View Article : Google Scholar : PubMed/NCBI
14	Hershey GK: IL-13 receptors and signaling pathways: an evolving web. J Allergy Clin Immunol. 111:677–691. 2003. View Article : Google Scholar : PubMed/NCBI
15	Rankin JA, Picarella DE, Geba GP, Temann UA, Prasad B, DiCosmo B, Tarallo A, Stripp B, Whitsett J and Flavell RA: Phenotypic and physiologic characterization of transgenic mice expressing interleukin 4 in the lung: lymphocytic and eosinophilic inflammation without airway hyperreactivity. Proc Natl Acad Sci USA. 93:7821–7825. 1996. View Article : Google Scholar : PubMed/NCBI
16	Wills-Karp M, Luyimbazi J, Xu X, Schofield B, Neben TY, Karp CL and Donaldson DD: Interleukin-13: central mediator of allergic asthma. Science. 282:2258–2261. 1998. View Article : Google Scholar : PubMed/NCBI
17	Zhu Z, Homer RJ, Wang Z, Chen Q, Geba GP, Wang J, Zhang Y and Elias JA: Pulmonary expression of interleukin-13 causes inflammation, mucus hypersecretion, subepithelial fibrosis, physiologic abnormalities, and eotaxin production. J Clin Invest. 103:779–788. 1999. View Article : Google Scholar : PubMed/NCBI
18	Rincon M and Irvin CG: Role of IL-6 in asthma and other inflammatory pulmonary diseases. Int J Biol Sci. 8:1281–1290. 2012. View Article : Google Scholar : PubMed/NCBI
19	Hamid Q, Shannon J and Martin J: Physiologic basis of respiratory disease. Cytokines and Chemokines in Asthma: An Overview. Tulic MK, Fiset PO, Muller Z and Hamid Q: BC Decker Inc; Hamilton: pp. 453–467. 2005
20	Bosnjak B, Stelzmueller B, Erb KJ and Epstein MM: Treatment of allergic asthma: modulation of Th2 cells and their responses. Respir Res. 12:1142011. View Article : Google Scholar : PubMed/NCBI
21	Barnes PJ: Current issues for establishing inhaled corticosteroids as the antiinflammatory agents of choice in asthma. J Allergy Clin Immunol. 101:S427–S433. 1998. View Article : Google Scholar : PubMed/NCBI
22	Wise J: Corticosteroids for asthma may suppress growth in children in first year of treatment, researchers say. BMJ. 349:g46232014. View Article : Google Scholar : PubMed/NCBI
23	Ciriaco M, Ventrice P, Russo G, Scicchitano M, Mazzitello G, Scicchitano F and Russo E: Corticosteroid-related central nervous system side effects. J Pharmacol Pharmacother. 4(Suppl 1): S94–S98. 2013. View Article : Google Scholar : PubMed/NCBI
24	Kwon Soon-Kyung: Erythronium japonicum. Yakup: http://www.yakup.com/pharmplus/plus_print.html?nid=3000131250. Last updated March 19, 2014.
25	Heo BG, Park YS, Chon SU, Lee SY, Cho JY and Gorinstein S: Antioxidant activity and cytotoxicity of methanol extracts from aerial parts of Korean salad plants. Biofactors. 30:79–89. 2007. View Article : Google Scholar
26	Boerjan W, Ralph J and Baucher M: Lignin biosynthesis. Annu Rev Plant Biol. 54:519–546. 2003. View Article : Google Scholar : PubMed/NCBI
27	Koriem KM and Soliman RE: Chlorogenic and caftaric acids in liver toxicity and oxidative stress induced by methamphetamine. J Toxicol. 2014:5834942014. View Article : Google Scholar : PubMed/NCBI
28	Revuelta-Iniesta R and Al-Dujaili EA: Consumption of green coffee reduces blood pressure and body composition by influencing 11β-HSD1 enzyme activity in healthy individuals: a pilot crossover study using green and black coffee. BioMed Res Int. 2014:4827042014. View Article : Google Scholar
29	Kim HR, Lee DM, Lee SH, Seong AR, Gin DW, Hwang JA and Park JH: Chlorogenic acid suppresses pulmonary eosinophilia, IgE production, and Th2-type cytokine production in an ovalbumin-induced allergic asthma: activation of STAT-6 and JNK is inhibited by chlorogenic acid. Int Immunopharmacol. 10:1242–1248. 2010. View Article : Google Scholar : PubMed/NCBI
30	Sy LB, Yang LK, Chiu CJ and Wu WM: The immunoregulatory effects of caffeic acid phenethyl ester on the cytokine secretion of peripheral blood mononuclear cells from asthmatic children. Pediatr Neonatol. 52:327–331. 2011. View Article : Google Scholar : PubMed/NCBI
31	Larché M, Robinson DS and Kay AB: The role of T lymphocytes in the pathogenesis of asthma. J Allergy Clin Immunol. 111:450–463; quiz 464. 2003. View Article : Google Scholar : PubMed/NCBI
32	Seo JW1, Cho SC, Park SJ, Lee EJ, Lee JH, Han SS, Pyo BS, Park DH and Kim BH: 1′-Acetoxychavicol acetate isolated from Alpinia galanga ameliorates ovalbumin-induced asthma in mice. PLosOne. 8:e564472013. View Article : Google Scholar
33	Bang MA, Seo JH, Seo JW, Jo GH, Jung SK, Yu R, Park DH and Park SJ: Bacillus subtilis KCTC 11782BP-produced alginate oligosaccharide effectively suppresses asthma via T-helper cell type 2-related cytokines. PLoS One. 10:e01175242015. View Article : Google Scholar : PubMed/NCBI