Open Access

Interleukin-6 and rs1800796 locus single nucleotide polymorphisms in response to hypoxia/reoxygenation in hepatocytes

  • Authors:
    • Zhaowen Wang
    • Shaohan Wu
    • Jianhua Liao
    • Lin Zhong
    • Tonghai Xing
    • Junwei Fan
    • Zhihai Peng
  • View Affiliations

  • Published online on: May 18, 2016     https://doi.org/10.3892/ijmm.2016.2595
  • Pages: 192-200
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ischemia-reperfusion injury due to hypoxia/reoxygenation (H/R) is one of the main causes of liver damage during liver surgery. Donor interleukin-6 (IL-6) rs1800796 single nucleotide polymorphisms (SNPs) affect the metabolism of tacrolimus following liver transplantation-related hepatic H/R. This study investigated the response of IL-6 and its promoter polymorphisms to hepatic H/R in liver parenchymal cells. The association between IL-6 rs1800796 SNPs and IL‑6 expression was measured in 84 disease-free liver tissues using tissue microarrays and immunohistochemistry. Subsequently, LO2G, LO2C and NC-LO2 cells were successfully constructed via stable lentivirus-mediated transfection. The effects of IL-6 and its SNPs on the biological function of LO2 cells were examined using a cell model of H/R. Our results revealed that IL-6 was mainly expressed in hepatocytes. The intermediate IL-6 expression rate in genotype CC carriers was higher than that in genotype CG/GG carriers (P=0.006), which was subsequently verified at the IL-6 mRNA level (P=0.002). The concentrations of alanine aminotransferase in the LO2G cells were significantly higher than those in the LO2C cells following H/R for 6 h and H/R for 24 h (P<0.05). The viability of the LO2C cells was higher than that of the LO2G cells (P<0.05). Furthermore, the expression of IL-6 and its downstream molecules was significantly increased in the LO2C cells compared with the LO2G cells (P<0.05). Therefore, the sequence variants of rs1800796 SNPs (G→C) exhibit an increased IL-6 transcription efficiency in liver parenchymal cells. In addition, the increased expression of IL-6 protects the hepatocytes following hepatic H/R injury.
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July-2016
Volume 38 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang Z, Wu S, Liao J, Zhong L, Xing T, Fan J and Peng Z: Interleukin-6 and rs1800796 locus single nucleotide polymorphisms in response to hypoxia/reoxygenation in hepatocytes. Int J Mol Med 38: 192-200, 2016
APA
Wang, Z., Wu, S., Liao, J., Zhong, L., Xing, T., Fan, J., & Peng, Z. (2016). Interleukin-6 and rs1800796 locus single nucleotide polymorphisms in response to hypoxia/reoxygenation in hepatocytes. International Journal of Molecular Medicine, 38, 192-200. https://doi.org/10.3892/ijmm.2016.2595
MLA
Wang, Z., Wu, S., Liao, J., Zhong, L., Xing, T., Fan, J., Peng, Z."Interleukin-6 and rs1800796 locus single nucleotide polymorphisms in response to hypoxia/reoxygenation in hepatocytes". International Journal of Molecular Medicine 38.1 (2016): 192-200.
Chicago
Wang, Z., Wu, S., Liao, J., Zhong, L., Xing, T., Fan, J., Peng, Z."Interleukin-6 and rs1800796 locus single nucleotide polymorphisms in response to hypoxia/reoxygenation in hepatocytes". International Journal of Molecular Medicine 38, no. 1 (2016): 192-200. https://doi.org/10.3892/ijmm.2016.2595