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Knockdown of HMGN2 increases the internalization of Klebsiella pneumoniae by respiratory epithelial cells through the regulation of α5β1 integrin expression

  • Authors:
    • Xinyuan Wang
    • Jingyu Li
    • Shanze Chen
    • Xiaofei Shen
    • Xiaolong Yang
    • Yan Teng
    • Luxia Deng
    • Yi Wang
    • Junli Chen
    • Xiaoying Wang
    • Ning Huang

  • View Affiliations

  • Published online on: July 25, 2016     https://doi.org/10.3892/ijmm.2016.2690
  • Pages: 737-746

  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Integrin receptors, a large family of adhesion receptors, are involved in the attachment of Klebsiella pneumoniae to respiratory epithelial cells, and subsequently cause the internalization of K. pneumoniae by host cells. Although a number of molecules have been reported to regulate the expression and activity of integrin receptors in respiratory epithelial cells, the specific underlying molecular mechanisms remain largely unknown. High mobility group nucleosomal binding domain 2 (HMGN2), a non-histone nuclear protein, is present in eukaryotic cells as a ubiquitous nuclear protein. Our previous studies have demonstrated that HMGN2 affects chromatin function and modulates the expression of antibacterial peptide in A549 cells exposed to lipopolysaccharide, which indicates the critical role of HMGN2 in innate immune responses. In addition, our cDNA microarray analysis suggested that HMGN2 knockdown induced the enhanced expression of α5β1 integrin in A549 cells. Therefore, we hypothesized that intercellular HMGN2 may mediate the internalization of K. pneumoniae by altering the expression of α5β1 integrin. Using the A549 cell line, we demonstrated that HMGN2 knockdown induced the increased expression of α5β1 integrin on cell membranes, which resulted in a significant increase in K. pneumoniae internalization. Further results revealed that HMGN2 silencing induced the expression of talin and the activation of α5β1 integrin, which led to actin polymerization following the phosphorylation of FAK and Src. This study suggests a possible therapeutic application for bacterial internalization by targeting HMGN2 in order to treat K. pneumoniae infection.
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September-2016
Volume 38 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • CiteScore: 10.7
  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
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Spandidos Publications style
Wang X, Li J, Chen S, Shen X, Yang X, Teng Y, Deng L, Wang Y, Chen J, Wang X, Wang X, et al: Knockdown of HMGN2 increases the internalization of Klebsiella pneumoniae by respiratory epithelial cells through the regulation of α5β1 integrin expression. Int J Mol Med 38: 737-746, 2016
APA
Wang, X., Li, J., Chen, S., Shen, X., Yang, X., Teng, Y. ... Huang, N. (2016). Knockdown of HMGN2 increases the internalization of Klebsiella pneumoniae by respiratory epithelial cells through the regulation of α5β1 integrin expression. International Journal of Molecular Medicine, 38, 737-746. https://doi.org/10.3892/ijmm.2016.2690
MLA
Wang, X., Li, J., Chen, S., Shen, X., Yang, X., Teng, Y., Deng, L., Wang, Y., Chen, J., Wang, X., Huang, N."Knockdown of HMGN2 increases the internalization of Klebsiella pneumoniae by respiratory epithelial cells through the regulation of α5β1 integrin expression". International Journal of Molecular Medicine 38.3 (2016): 737-746.
Chicago
Wang, X., Li, J., Chen, S., Shen, X., Yang, X., Teng, Y., Deng, L., Wang, Y., Chen, J., Wang, X., Huang, N."Knockdown of HMGN2 increases the internalization of Klebsiella pneumoniae by respiratory epithelial cells through the regulation of α5β1 integrin expression". International Journal of Molecular Medicine 38, no. 3 (2016): 737-746. https://doi.org/10.3892/ijmm.2016.2690