Open Access

HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line

  • Authors:
    • Xiaofeng Zhu
    • Shuichuan Huang
    • Lili Zeng
    • Jieyi Ma
    • Suhong Sun
    • Feng Zeng
    • Fanli Kong
    • Xiaoming Cheng
  • View Affiliations

  • Published online on: June 14, 2017     https://doi.org/10.3892/ijmm.2017.3027
  • Pages: 411-417
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Epithelial‑mesenchymal transition (EMT) is a key mechanism underlying metastatic breast cancer. Reactive oxygen species (ROS) play an important role in EMT. Heme oxygenase‑1 (HMOX‑1) can reduce oxidative stress. However, the effect of HMOX‑1 on the EMT process in breast cancer cells is unknown. We treated the MCF‑7 breast cancer cell line with the HMOX‑1 inducer hemin and observed that hemin induced HMOX‑1 expression and inhibited migration, invasion and ROS generation in transforming growth factor‑β (TGF‑β)‑treated MCF‑7 cells using quantitative RT‑qPCR, western blotting, wound‑healing and cell invasion assays as well as fluorescent probe DCFDA. Hemin inhibited TGF‑β‑induced EMT in the MCF‑7 cells, whereas HMOX‑1 siRNA attenuated the suppressive effect of hemin as determined by the expression and cellular distribution of selected EMT markers. In summary, our results revealed that hemin treatment increased HMOX‑1 expression and inhibited TGF‑β‑induced EMT in MCF‑7 cells.
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August-2017
Volume 40 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhu X, Huang S, Zeng L, Ma J, Sun S, Zeng F, Kong F and Cheng X: HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line. Int J Mol Med 40: 411-417, 2017
APA
Zhu, X., Huang, S., Zeng, L., Ma, J., Sun, S., Zeng, F. ... Cheng, X. (2017). HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line. International Journal of Molecular Medicine, 40, 411-417. https://doi.org/10.3892/ijmm.2017.3027
MLA
Zhu, X., Huang, S., Zeng, L., Ma, J., Sun, S., Zeng, F., Kong, F., Cheng, X."HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line". International Journal of Molecular Medicine 40.2 (2017): 411-417.
Chicago
Zhu, X., Huang, S., Zeng, L., Ma, J., Sun, S., Zeng, F., Kong, F., Cheng, X."HMOX-1 inhibits TGF-β-induced epithelial-mesenchymal transition in the MCF-7 breast cancer cell line". International Journal of Molecular Medicine 40, no. 2 (2017): 411-417. https://doi.org/10.3892/ijmm.2017.3027