JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts

Song,Qin; Xie,Yuxin; Gou,Qiheng; Guo,Xiaoqiang; Yao,Qian; Gou,Xiaojun

doi:10.3892/ijmm.2017.3040

JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts

Authors:
- Qin Song
- Yuxin Xie
- Qiheng Gou
- Xiaoqiang Guo
- Qian Yao
- Xiaojun Gou
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Affiliations: College of Pharmacy and Bioengineering, Chengdu University, Chengdu, Sichuan 610106, P.R. China, Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: June 26, 2017 https://doi.org/10.3892/ijmm.2017.3040
Pages: 465-473
Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Periplaneta americana extracts (PAEs) play a crucial role in skin wound healing. However, their molecular effects and signaling pathways in regenerating tissues and cells are not clear. In this study, we refined the PAE from Periplaneta americana to investigate the mechanisms underlying skin wound healing. The human keratinocyte line HaCaT was selected and a mouse model of deep second-degree thermal burn was established for in vitro and in vivo studies, respectively. PAE treatment induced the proliferation and migration of HaCaT cells and wound healing in the burn model. Furthermore, the effects of PAE on wound healing were found to depend on the Janus-activated kinase/signal transducer and activator of transcription 3 (JAK/STAT3) pathway and Smad3 activities, according to western blot analysis and immunohistochemical (IHC) assays in vitro and in vivo. Pretreatment with a STAT3 inhibitor blocked the cell proliferation and migration induced by PAE. The results indicate the wound-healing function of PAE via enhanced JAK/STAT3 signaling and Smad3 activities. Our studies provide a theoretical basis underlying the role of PAE in cutaneous wound healing.

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