Open Access

EWS-FLI1 positively regulates autophagy by increasing ATG4B expression in Ewing sarcoma cells

  • Authors:
    • Qunshan Lu
    • Yuankai Zhang
    • Liang Ma
    • Deqiang Li
    • Ming Li
    • Jianmin Li
    • Peilai Liu
  • View Affiliations

  • Published online on: August 29, 2017     https://doi.org/10.3892/ijmm.2017.3112
  • Pages: 1217-1225
  • Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ewing sarcoma (ES) is the most common malignant bone tumor in children and young adults. It is characterized by chromosomal translocations fusing the EWS gene with an ETS oncogene, most frequently FLI1. In the present study, the authors aimed to investigate the function of EWS-FLI1 in autophagy in ES cells, and identified that EWS-FLI1 positively regulates autophagy in ES cells. ATG4B expression was observed markedly upregulated by EWS-FLI1 overexpression, and silencing of ATG4B dramatically inhibits autophagy in ES cells. Furthermore, apoptosis was inhibited in ATG4B overexpressed ES cells, and ATG4B-potentiated autophagy is required for ES cells survival. Taken together, the authors demonstrated the role of EWS-FLI1 and ATG4B in autophagy in ES cells, and suggested EWS-FLI1 and ATG4B as potential therapeutic targets for ES.
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October-2017
Volume 40 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lu Q, Zhang Y, Ma L, Li D, Li M, Li J and Liu P: EWS-FLI1 positively regulates autophagy by increasing ATG4B expression in Ewing sarcoma cells. Int J Mol Med 40: 1217-1225, 2017
APA
Lu, Q., Zhang, Y., Ma, L., Li, D., Li, M., Li, J., & Liu, P. (2017). EWS-FLI1 positively regulates autophagy by increasing ATG4B expression in Ewing sarcoma cells. International Journal of Molecular Medicine, 40, 1217-1225. https://doi.org/10.3892/ijmm.2017.3112
MLA
Lu, Q., Zhang, Y., Ma, L., Li, D., Li, M., Li, J., Liu, P."EWS-FLI1 positively regulates autophagy by increasing ATG4B expression in Ewing sarcoma cells". International Journal of Molecular Medicine 40.4 (2017): 1217-1225.
Chicago
Lu, Q., Zhang, Y., Ma, L., Li, D., Li, M., Li, J., Liu, P."EWS-FLI1 positively regulates autophagy by increasing ATG4B expression in Ewing sarcoma cells". International Journal of Molecular Medicine 40, no. 4 (2017): 1217-1225. https://doi.org/10.3892/ijmm.2017.3112