Open Access

Transcription factors Nrf2 and NF-κB contribute to inflammation and apoptosis induced by intestinal ischemia-reperfusion in mice

  • Authors:
    • Qing-Tao Meng
    • Rong Chen
    • Cheng Chen
    • Ke Su
    • Wei Li
    • Ling-Hua Tang
    • Hui-Min Liu
    • Rui Xue
    • Qian Sun
    • Yan Leng
    • Jia-Bao Hou
    • Yang Wu
    • Zhong-Yuan Xia
  • View Affiliations

  • Published online on: October 2, 2017     https://doi.org/10.3892/ijmm.2017.3170
  • Pages:1731-1740
  • Copyright: © Meng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Intestinal ischemia/reperfusion (IIR) is a common pathological event associated with intestinal injury and apoptosis with high mortality. Nuclear factor (NF)-E2-related factor-2 (Nrf2) is a key transcription factor that interacts with NF-κB and has a vital anti-inflammatory effect. However, whether Nrf2 has a role in IIR-induced apoptosis and the possible underlining mechanisms, such as modulation of the inflammation regulation pathway, have remained to be fully elucidated. In the present study, IIR was identified to cause significant intestinal injury and apoptosis, with high expression levels of inflammatory cytokines, as well as the apoptotic proteins B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax) and caspase-3, while simultaneously decreasing the protein levels of Bcl-2. The effect was more pronounced after pretreatment of the animals with all-trans retinoic acid or brusatol, potent inhibitors of Nrf2. t-Butylhydroquinone, an Nrf2 activator, significantly attenuated IIR-induced intestinal injury and apoptosis, with inhibition of the overexpression of the inflammatory cytokines, Bax and caspase-3 protein and partial restoration of Bcl-2 protein expression. Taken together, these results indicated that increased Nrf2 expression reduced IIR-induced intestinal apoptosis and that the protective function of Nrf2 may be based on its anti-inflammatory effects through the inhibition of the NF-κB pathway.

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December 2017
Volume 40 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
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APA
Meng, Q., Chen, R., Chen, C., Su, K., Li, W., Tang, L. ... Xia, Z. (2017). Transcription factors Nrf2 and NF-κB contribute to inflammation and apoptosis induced by intestinal ischemia-reperfusion in mice. International Journal of Molecular Medicine, 40, 1731-1740. https://doi.org/10.3892/ijmm.2017.3170
MLA
Meng, Q., Chen, R., Chen, C., Su, K., Li, W., Tang, L., Liu, H., Xue, R., Sun, Q., Leng, Y., Hou, J., Wu, Y., Xia, Z."Transcription factors Nrf2 and NF-κB contribute to inflammation and apoptosis induced by intestinal ischemia-reperfusion in mice". International Journal of Molecular Medicine 40.6 (2017): 1731-1740.
Chicago
Meng, Q., Chen, R., Chen, C., Su, K., Li, W., Tang, L., Liu, H., Xue, R., Sun, Q., Leng, Y., Hou, J., Wu, Y., Xia, Z."Transcription factors Nrf2 and NF-κB contribute to inflammation and apoptosis induced by intestinal ischemia-reperfusion in mice". International Journal of Molecular Medicine 40, no. 6 (2017): 1731-1740. https://doi.org/10.3892/ijmm.2017.3170