Protective effects of neem (Azadirachta indica A. Juss.) leaf extract against cigarette smoke- and lipopolysaccharide-induced pulmonary inflammation

  • Authors:
    • Jae-Won Lee
    • Hyung Won Ryu
    • So-Yeon Park
    • Hyun Ah Park
    • Ok-Kyoung Kwon
    • Heung Joo Yuk
    • Krishna K. Shrestha
    • Minwoo Park
    • Jung Hee Kim
    • Sangwoo Lee
    • Sei-Ryang Oh
    • Kyung-Seop Ahn
  • View Affiliations

  • Published online on: October 10, 2017     https://doi.org/10.3892/ijmm.2017.3178
  • Pages:1932-1940
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Abstract

Neem (Azadirachta indica A. Juss.) leaf has been reported to exert anti-inflammatory, antibacterial and antioxidant effects. The purpose of this study was to investigate the protective effects of neem leaf extract (NLE) against cigarette smoke (CS)- and lipopolysaccharide (LPS)-induced pulmonary inflammation. Treatment with NLE significantly attenuated the infiltration of inflammatory cells, such as neutrophils and macrophages in bronchoalveolar lavage fluid (BALF). NLE also reduced the production of reactive oxygen species and the activity of neutrophil elastase in BALF. Moreover, NLE attenuated the release of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in BALF. NLE inhibited the recruitment of inflammatory cells and the expression of monocyte chemoattractant protein-1 (MCP-1) in the lungs of mice with CS- and LPS-induced pulmonary inflammation. NLE also decreased the expression of inducible nitric oxide synthase (iNOS) in the lungs of the mice CS- and LPS-induced pulmonary inflammation. Furthermore, treatment with NLE significantly attenuated the activation of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) in the lungs mice exposed to CS and LPS. NLE also inhibited the phosphorylation of nuclear factor (NF)-κB and inhibitor of NF-κB (IκB) in the lungs of mice expose to CS and LPS. These findings thus suggest that NLE has potential for use in the treatment of chronic obstructive pulmonary disease.

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December 2017
Volume 40 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Lee, J., Ryu, H.W., Park, S., Park, H.A., Kwon, O., Yuk, H.J. ... Ahn, K. (2017). Protective effects of neem (Azadirachta indica A. Juss.) leaf extract against cigarette smoke- and lipopolysaccharide-induced pulmonary inflammation. International Journal of Molecular Medicine, 40, 1932-1940. https://doi.org/10.3892/ijmm.2017.3178
MLA
Lee, J., Ryu, H. W., Park, S., Park, H. A., Kwon, O., Yuk, H. J., Shrestha, K. K., Park, M., Kim, J. H., Lee, S., Oh, S., Ahn, K."Protective effects of neem (Azadirachta indica A. Juss.) leaf extract against cigarette smoke- and lipopolysaccharide-induced pulmonary inflammation". International Journal of Molecular Medicine 40.6 (2017): 1932-1940.
Chicago
Lee, J., Ryu, H. W., Park, S., Park, H. A., Kwon, O., Yuk, H. J., Shrestha, K. K., Park, M., Kim, J. H., Lee, S., Oh, S., Ahn, K."Protective effects of neem (Azadirachta indica A. Juss.) leaf extract against cigarette smoke- and lipopolysaccharide-induced pulmonary inflammation". International Journal of Molecular Medicine 40, no. 6 (2017): 1932-1940. https://doi.org/10.3892/ijmm.2017.3178