Insulin restores UCP3 activity and decreases energy surfeit to alleviate lipotoxicity in skeletal muscle

  • Authors:
    • Wenjuan Tang
    • Sunyinyan Tang
    • Hongdong Wang
    • Zhijuan Ge
    • Dalong Zhu
    • Yan Bi
  • View Affiliations

  • Published online on: October 2, 2017     https://doi.org/10.3892/ijmm.2017.3169
  • Pages:2000-2010
0

Abstract

An early insulin regimen ameliorates glucotoxicity but also lipotoxicity in type 2 diabetes; however, the underlying mechanism remains elusive. In the present study, we investigated the role of mitochondria in lipid regulation following early insulin administration in insulin-resistant skeletal muscle cells. Male C57BL/6 mice, fed a high-fat diet (HFD) for 8 weeks, were treated with insulin for 3 weeks, and L6 myotubes cultured with palmitate (PA) for 24 h were incubated with insulin for another 12 h. The results showed that insulin facilitated systemic glucose disposal and attenuated muscular triglyceride accumulation in vivo. Recovery of AMP-activated protein kinase (AMPK) phosphorylation, inhibition of sterol-regulated element binding protein-1c (SREBP-1c) and increased carnitine palmitoyltransferase‑1B (CPT1B) expression were observed after insulin administration. Moreover, increased ATP concentration and cellular energy charge elicited by over-nutrition were suppressed by insulin. Despite maintaining respiratory complex activities, insulin restored muscular uncoupling protein 3 (UCP3) protein expression in vitro and in vivo. By contrast, knockdown of UCP3 abrogated insulin-induced restoration of AMPK phosphorylation in vitro. Importantly, the PA-induced decrease in UCP3 was blocked by the proteasome inhibitor MG132, and insulin reduced UCP3 ubiquitination, thereby prohibiting its degradation. Our findings, focusing on energy balance, provide a mechanistic understanding of the promising effect of early insulin initiation on lipotoxicity. Insulin, by recovering UCP3 activity, alleviated energy surfeit and potentiated AMPK-mediated lipid homeostasis in skeletal muscle cells following exposure to PA and in gastrocnemius of mice fed HFD.

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December 2017
Volume 40 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Tang, W., Tang, S., Wang, H., Ge, Z., Zhu, D., & Bi, Y. (2017). Insulin restores UCP3 activity and decreases energy surfeit to alleviate lipotoxicity in skeletal muscle. International Journal of Molecular Medicine, 40, 2000-2010. https://doi.org/10.3892/ijmm.2017.3169
MLA
Tang, W., Tang, S., Wang, H., Ge, Z., Zhu, D., Bi, Y."Insulin restores UCP3 activity and decreases energy surfeit to alleviate lipotoxicity in skeletal muscle". International Journal of Molecular Medicine 40.6 (2017): 2000-2010.
Chicago
Tang, W., Tang, S., Wang, H., Ge, Z., Zhu, D., Bi, Y."Insulin restores UCP3 activity and decreases energy surfeit to alleviate lipotoxicity in skeletal muscle". International Journal of Molecular Medicine 40, no. 6 (2017): 2000-2010. https://doi.org/10.3892/ijmm.2017.3169