Open Access

Radical scavenging-linked anti-adipogenic activity of Alnus firma extracts

  • Authors:
    • Sun-Il Choi
    • Jong Seok Lee
    • Sarah Lee
    • Jin-Ha Lee
    • Hee-Sun Yang
    • Joohong Yeo
    • Jong-Yea Kim
    • Boo-Yong Lee
    • Il-Jun Kang
    • Ok-Hwan Lee
  • View Affiliations

  • Published online on: October 27, 2017     https://doi.org/10.3892/ijmm.2017.3221
  • Pages:119-128
  • Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The purpose of the present study was to investigate the antioxidant activity and anti-adipogenic effect of extracts from Alnus firma (A. firma), which is an edible plant that grows in mountainous areas. The total phenolic, flavonoid and anthocyanin content as well as the antioxidant activity of a 70% ethanolic extract of A. firma (AFE) was assessed. Furthermore, the effects of AFE on lipid accumulation and reactive oxygen species (ROS) production during adipogenesis of 3T3-L1 cells were investigated. The results revealed that the total phenolic, flavonoid and pro-anthocyanidin content of AFE as 436.26±3.30 mg gallic acid equivalents/g, 73.82±0.54 mg quercetin equivalents/g and 149.25±6.06 mg catechin equivalents/g, respectively. In addition, AFE exerted significant antioxidant effects in terms of 1,1-diphenyl-2-picryl hydrazyl radical scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, reducing power, oxygen radical absorbance capacity and nitric oxide radical scavenging activity. As for its anti-adipogenic activity, AFE significantly inhibited ROS production and lipid accumulation during adipogenesis of 3T3-L1 cells compared with those in control cells. In addition, AFE regulated adipogenic transcription factors including peroxisome proliferator‑activated receptor-γ, CCAAT/enhance-binding protein α and adipocyte protein 2. These results indicated that A. firma is a potential candidate for a functional food supplement.

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January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Choi, S., Lee, J.S., Lee, S., Lee, J., Yang, H., Yeo, J. ... Lee, O. (2018). Radical scavenging-linked anti-adipogenic activity of Alnus firma extracts. International Journal of Molecular Medicine, 41, 119-128. https://doi.org/10.3892/ijmm.2017.3221
MLA
Choi, S., Lee, J. S., Lee, S., Lee, J., Yang, H., Yeo, J., Kim, J., Lee, B., Kang, I., Lee, O."Radical scavenging-linked anti-adipogenic activity of Alnus firma extracts". International Journal of Molecular Medicine 41.1 (2018): 119-128.
Chicago
Choi, S., Lee, J. S., Lee, S., Lee, J., Yang, H., Yeo, J., Kim, J., Lee, B., Kang, I., Lee, O."Radical scavenging-linked anti-adipogenic activity of Alnus firma extracts". International Journal of Molecular Medicine 41, no. 1 (2018): 119-128. https://doi.org/10.3892/ijmm.2017.3221