Open Access

Synovial fluid‑derived synovial fragments represent an improved source of synovial mesenchymal stem cells in the temporomandibular joint

  • Authors:
    • Yu Yao
    • Zheng‑Yu Li
    • Hong Zhang
    • You‑Hua Zheng
    • Li‑Xiang Mai
    • Wen‑Jing Liu
    • Zhi‑Guang Zhang
    • Yang‑Peng Sun
  • View Affiliations

  • Published online on: October 25, 2017     https://doi.org/10.3892/ijmm.2017.3210
  • Pages:173-183
  • Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Surgery-obtained synovium specimens (SSSs) can provide a source of synovial mesenchymal stem cells (SMSCs) for experimental studies. However, these specimens contain diverse tissues, including the intima and subintima; therefore, these SMSCs are not entirely derived from the intima and their cell source is heterogeneous. The present study isolated synovial fragments (SFs) from synovial fluid dilutions extracted from patients with temporomandibular joint (TMJ) osteoarthrosis. Unlike SSSs, SFs, which are membranous and translucent, consist of only several cell layers, indicating the presence of only the intima. In the present study, SF cells (SFCs) and SSS cells (SSSCs) exhibited a homogeneous, fibroblast‑like, spindle‑shaped morphology after passaging in vitro. Furthermore, both cell types exhibited similar proliferative and differentiation potentials in vitro. However, SFCs exhibited more uniform surface markers compared with SSSCs when analysed by flow cytometry. Taken together, these results indicated that SFs contained a greater amount of unmixed intima than SSSs, and that SFCs exhibited more homogeneous characteristics than SSSCs, thereby offering an improved source of SMSCs in the TMJ.

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January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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Copy and paste a formatted citation
APA
Yao, Y., Li, Z., Zhang, H., Zheng, Y., Mai, L., Liu, W. ... Sun, Y. (2018). Synovial fluid‑derived synovial fragments represent an improved source of synovial mesenchymal stem cells in the temporomandibular joint. International Journal of Molecular Medicine, 41, 173-183. https://doi.org/10.3892/ijmm.2017.3210
MLA
Yao, Y., Li, Z., Zhang, H., Zheng, Y., Mai, L., Liu, W., Zhang, Z., Sun, Y."Synovial fluid‑derived synovial fragments represent an improved source of synovial mesenchymal stem cells in the temporomandibular joint". International Journal of Molecular Medicine 41.1 (2018): 173-183.
Chicago
Yao, Y., Li, Z., Zhang, H., Zheng, Y., Mai, L., Liu, W., Zhang, Z., Sun, Y."Synovial fluid‑derived synovial fragments represent an improved source of synovial mesenchymal stem cells in the temporomandibular joint". International Journal of Molecular Medicine 41, no. 1 (2018): 173-183. https://doi.org/10.3892/ijmm.2017.3210