In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats

Liu,Qian; Zhang,Xiaoxia; Jiao,Yang; Liu,Xin; Wang,Yirong; Li,Song-Lun; Zhang,Wei; Chen,Fa-Ming; Ding,Yin; Jiang,Chuan; Jin,Zuolin

doi:10.3892/ijmm.2017.3280

In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats

Authors:
- Qian Liu
- Xiaoxia Zhang
- Yang Jiao
- Xin Liu
- Yirong Wang
- Song-Lun Li
- Wei Zhang
- Fa-Ming Chen
- Yin Ding
- Chuan Jiang
- Zuolin Jin
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Affiliations: State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Stomatology, Xi’an Medical College, Xi'an, Shaanxi 710309, P.R. China, Department of Stomatology, PLA Army General Hospital, Beijing 100700, P.R. China, State Key Laboratory of Military Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Medical Service Management Office, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Research Center of Traditional Chinese Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Stomatology, The People's Hospital of Tongchuan, Tongchuan 727000, P.R. China
Published online on: November 22, 2017 https://doi.org/10.3892/ijmm.2017.3280
Pages: 669-678
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Postmenopausal osteoporosis (PMO) increases bone fragility and the risk of fractures, and impairs the healing procedure of bone defects in aged women. The stromal cell‑derived factor-1α (SDF-1α)/CXC chemokine receptor type 4 (CXCR4) axis helps to maintain the biological and physiological functions of bone marrow mesenchymal stem cells (BMSCs) and increase the homing efficiency of BMSCs. The present study aimed to provide insights into the possible association between migration and osteogenic ability and the SDF-1α/CXCR4 axis in BMSCs derived from a rat model of PMO. In order to do this, the general and SDF-1α/CXCR4-associated biological characteristics as well as associated molecular mechanisms in BMSCs isolated from a PMO rat model (OVX-BMSCs) and normal rats (Sham‑BMSCs) were investigated and compared. In comparison with Sham-BMSCs, OVX-BMSCs exhibited an impaired osteogenic ability, but a stronger adipogenic activity as well as a higher proliferative ability. In addition, OVX-BMSCs presented a lower chemotactic activity towards SDF-1α, lower expression levels of CXCR4 and reduced levels of phosphorylated AKT (p-AKT). Therefore, the lower expression levels of CXCR4 and p-AKT may be responsible for the impaired osteogenic ability and lower chemotactic activity towards SDF-1α of OVX-BMSCs.

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