Open Access

In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats

  • Authors:
    • Qian Liu
    • Xiaoxia Zhang
    • Yang Jiao
    • Xin Liu
    • Yirong Wang
    • Song-Lun Li
    • Wei Zhang
    • Fa-Ming Chen
    • Yin Ding
    • Chuan Jiang
    • Zuolin Jin
  • View Affiliations

  • Published online on: November 22, 2017     https://doi.org/10.3892/ijmm.2017.3280
  • Pages:669-678
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Postmenopausal osteoporosis (PMO) increases bone fragility and the risk of fractures, and impairs the healing procedure of bone defects in aged women. The stromal cell‑derived factor-1α (SDF-1α)/CXC chemokine receptor type 4 (CXCR4) axis helps to maintain the biological and physiological functions of bone marrow mesenchymal stem cells (BMSCs) and increase the homing efficiency of BMSCs. The present study aimed to provide insights into the possible association between migration and osteogenic ability and the SDF-1α/CXCR4 axis in BMSCs derived from a rat model of PMO. In order to do this, the general and SDF-1α/CXCR4-associated biological characteristics as well as associated molecular mechanisms in BMSCs isolated from a PMO rat model (OVX-BMSCs) and normal rats (Sham‑BMSCs) were investigated and compared. In comparison with Sham-BMSCs, OVX-BMSCs exhibited an impaired osteo­genic ability, but a stronger adipogenic activity as well as a higher proliferative ability. In addition, OVX-BMSCs presented a lower chemotactic activity towards SDF-1α, lower expression levels of CXCR4 and reduced levels of phosphorylated AKT (p-AKT). Therefore, the lower expression levels of CXCR4 and p-AKT may be responsible for the impaired osteogenic ability and lower chemotactic activity towards SDF-1α of OVX-BMSCs.

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February 2018
Volume 41 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
APA
Liu, Q., Zhang, X., Jiao, Y., Liu, X., Wang, Y., Li, S. ... Jin, Z. (2018). In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats. International Journal of Molecular Medicine, 41, 669-678. https://doi.org/10.3892/ijmm.2017.3280
MLA
Liu, Q., Zhang, X., Jiao, Y., Liu, X., Wang, Y., Li, S., Zhang, W., Chen, F., Ding, Y., Jiang, C., Jin, Z."In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats". International Journal of Molecular Medicine 41.2 (2018): 669-678.
Chicago
Liu, Q., Zhang, X., Jiao, Y., Liu, X., Wang, Y., Li, S., Zhang, W., Chen, F., Ding, Y., Jiang, C., Jin, Z."In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats". International Journal of Molecular Medicine 41, no. 2 (2018): 669-678. https://doi.org/10.3892/ijmm.2017.3280