Downregulation of adult and neonatal Nav1.5 in the dorsal root ganglia and axon of peripheral sensory neurons of rats with spared nerve injury

  • Authors:
    • Jun Wang
    • Shao-Wu Ou
    • Yun-Fei Bai
    • Yun-Jie Wang
    • Zhi-Qing David Xu
    • Guo-Ming Luan
  • View Affiliations

  • Published online on: January 30, 2018     https://doi.org/10.3892/ijmm.2018.3446
  • Pages: 2225-2232
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Abstract

Previous studies demonstrated that Nav1.5 splice variants, including Nav1.5a and Nav1.5c, were expressed in dorsal root ganglia (DRG) neurons. However, since nine Nav1.5 isoforms have been identified, whether other Nav1.5 splice variants, especially the neonatal Nav1.5 splice variant, express in the DRG neurons is still unknown. In this study, we systematically investigated the expression of adult and neonatal Nav1.5 isoforms in the DRG neurons and axon of peripheral sensory neurons of rats with spared nerve injury (SNI) by RT-PCR, DNA sequencing, restriction enzyme digestion, immunohistochemistry and immunofluorescence methods. The results demonstrated that both adult and neonatal Nav1.5 isoforms were expressed in the DRG neurons, but their expression ratio was ~2.5:1. In SNI rat models, the expression of both adult and neonatal Nav1.5 decreased by approximately a half in both mRNA and protein levels. In contrast, the expression of protein kinase C (PKC)-γ, one of the negative modulators for sodium currents, increased by ~1-fold. Taken together, this study first confirmed the expression of both adult and neonatal Nav1.5 isoforms in the DRG neurons and axon of peripheral sensory neurons of rat, but their expression level decreased in pain models. The upregulation of PKC-γ may directly or indirectly downregulate the expression of Nav1.5 isoforms in SNI rat models, which may further involve in the pathological process of neuropathic pain.
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April-2018
Volume 41 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang J, Ou S, Bai Y, Wang Y, Xu ZD and Luan G: Downregulation of adult and neonatal Nav1.5 in the dorsal root ganglia and axon of peripheral sensory neurons of rats with spared nerve injury. Int J Mol Med 41: 2225-2232, 2018
APA
Wang, J., Ou, S., Bai, Y., Wang, Y., Xu, Z.D., & Luan, G. (2018). Downregulation of adult and neonatal Nav1.5 in the dorsal root ganglia and axon of peripheral sensory neurons of rats with spared nerve injury. International Journal of Molecular Medicine, 41, 2225-2232. https://doi.org/10.3892/ijmm.2018.3446
MLA
Wang, J., Ou, S., Bai, Y., Wang, Y., Xu, Z. D., Luan, G."Downregulation of adult and neonatal Nav1.5 in the dorsal root ganglia and axon of peripheral sensory neurons of rats with spared nerve injury". International Journal of Molecular Medicine 41.4 (2018): 2225-2232.
Chicago
Wang, J., Ou, S., Bai, Y., Wang, Y., Xu, Z. D., Luan, G."Downregulation of adult and neonatal Nav1.5 in the dorsal root ganglia and axon of peripheral sensory neurons of rats with spared nerve injury". International Journal of Molecular Medicine 41, no. 4 (2018): 2225-2232. https://doi.org/10.3892/ijmm.2018.3446