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International Journal of Molecular Medicine
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Article

miR-195 suppresses abdominal aortic aneurysm through the TNF-α/NF-κB and VEGF/PI3K/Akt pathway

  • Authors:
    • Xiaohui Ma
    • Hairong Yao
    • Yuhong Yang
    • Long Jin
    • Yu Wang
    • Lina Wu
    • Shengli Yang
    • Kang Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, General Hospital of People's Liberation Army, Beijing 100853, P.R. China, Department of Cardiology, Xi'an No. 3 Hospital, Xi'an, Shaanxi 710018, P.R. China, Department of Cardiology, People's Hospital of Yuncheng, Yuncheng, Shanxi 044000, P.R. China, Department of Cardiology, Xi'an Ninth Hospital, Xi'an, Shaanxi 710004, P.R. China, Department of Nursing, Xi'an No. 3 Hospital, Xi'an, Shaanxi 710018, P.R. China, Department of Cardiology, Xi Jing Hospital, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 2350-2358
    |
    Published online on: January 25, 2018
       https://doi.org/10.3892/ijmm.2018.3426
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Abstract

In the present study, the function of microRNA (miR)‑195 on abdominal aortic aneurysm (AAA) and its possible mechanism were investigated. Reverse transcription‑quantitative polymerase chain reaction analysis was used to detect the expression of miR‑195 in patients with AAA. The expression levels of miR‑195 in patients with AAA were effectively increased. The present study also used miR‑195 mimics to increase the expression of miR‑195, and ELISA kits and western blot analysis were used to analyze the levels of interleukin (IL)‑1β and IL‑6, and the protein expression levels of matrix metalloproteinase (MMP)‑2, MMP‑9, tumor necrosis factor (TNF)‑α, nuclear factor (NF)‑κB, vascular endothelial growth factor (VEGF), phosphoinositide 3‑kinase (PI3K) and phosphorylated (p‑)Akt. The overexpression of miR‑195 promoted the levels of IL‑1β and IL‑6, induced the protein expression of MMP‑2 and MMP‑9, upregulated the protein expression of TNF‑α and NF‑κB, and suppressed the protein expression levels of VEGF, PI3K and p‑Akt in angiotensin II‑vascular smooth muscle cells. In addition, TNF‑α promoted the pre‑inflammatory effect of miR‑195 on the protein expression levels of TNF‑α and NF‑κB, levels of IL‑1β and IL‑6, and protein expression levels of MMP‑2 and MMP‑9 in the angiotensin II‑vascular smooth muscle cells. Suppression of PI3K promoted the pre‑inflammatory effect of miR‑195 on the protein expression of PI3K, p‑Akt and VEGF, the levels of IL‑1β and IL‑6, and the protein expression of MMP‑2 and MMP‑9 in angiotensin II‑vascular smooth muscle cells. Combined, these results suggested that miR‑195 suppressed AAA inflammation through the TNF‑α/NF‑κB and VEGF/PI3K/Akt pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Ma X, Yao H, Yang Y, Jin L, Wang Y, Wu L, Yang S and Cheng K: miR-195 suppresses abdominal aortic aneurysm through the TNF-α/NF-κB and VEGF/PI3K/Akt pathway. Int J Mol Med 41: 2350-2358, 2018.
APA
Ma, X., Yao, H., Yang, Y., Jin, L., Wang, Y., Wu, L. ... Cheng, K. (2018). miR-195 suppresses abdominal aortic aneurysm through the TNF-α/NF-κB and VEGF/PI3K/Akt pathway. International Journal of Molecular Medicine, 41, 2350-2358. https://doi.org/10.3892/ijmm.2018.3426
MLA
Ma, X., Yao, H., Yang, Y., Jin, L., Wang, Y., Wu, L., Yang, S., Cheng, K."miR-195 suppresses abdominal aortic aneurysm through the TNF-α/NF-κB and VEGF/PI3K/Akt pathway". International Journal of Molecular Medicine 41.4 (2018): 2350-2358.
Chicago
Ma, X., Yao, H., Yang, Y., Jin, L., Wang, Y., Wu, L., Yang, S., Cheng, K."miR-195 suppresses abdominal aortic aneurysm through the TNF-α/NF-κB and VEGF/PI3K/Akt pathway". International Journal of Molecular Medicine 41, no. 4 (2018): 2350-2358. https://doi.org/10.3892/ijmm.2018.3426
Copy and paste a formatted citation
x
Spandidos Publications style
Ma X, Yao H, Yang Y, Jin L, Wang Y, Wu L, Yang S and Cheng K: miR-195 suppresses abdominal aortic aneurysm through the TNF-α/NF-κB and VEGF/PI3K/Akt pathway. Int J Mol Med 41: 2350-2358, 2018.
APA
Ma, X., Yao, H., Yang, Y., Jin, L., Wang, Y., Wu, L. ... Cheng, K. (2018). miR-195 suppresses abdominal aortic aneurysm through the TNF-α/NF-κB and VEGF/PI3K/Akt pathway. International Journal of Molecular Medicine, 41, 2350-2358. https://doi.org/10.3892/ijmm.2018.3426
MLA
Ma, X., Yao, H., Yang, Y., Jin, L., Wang, Y., Wu, L., Yang, S., Cheng, K."miR-195 suppresses abdominal aortic aneurysm through the TNF-α/NF-κB and VEGF/PI3K/Akt pathway". International Journal of Molecular Medicine 41.4 (2018): 2350-2358.
Chicago
Ma, X., Yao, H., Yang, Y., Jin, L., Wang, Y., Wu, L., Yang, S., Cheng, K."miR-195 suppresses abdominal aortic aneurysm through the TNF-α/NF-κB and VEGF/PI3K/Akt pathway". International Journal of Molecular Medicine 41, no. 4 (2018): 2350-2358. https://doi.org/10.3892/ijmm.2018.3426
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