Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis

Ickrath,Pascal; Kleinsasser,Norbert; Ding,Xin; Ginzkey,Christian; Beyersdorf,Niklas; Hagen,Rudolf; Kerkau,Thomas; Hackenberg,Stephan

doi:10.3892/ijmm.2018.3653

Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis

Authors:
- Pascal Ickrath
- Norbert Kleinsasser
- Xin Ding
- Christian Ginzkey
- Niklas Beyersdorf
- Rudolf Hagen
- Thomas Kerkau
- Stephan Hackenberg
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Affiliations: Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Wuerzburg, D‑97080 Wuerzburg, Germany, Department of Otorhinolaryngology, Head and Neck Surgery, Kepler University Hospital, A‑4021 Linz, Austria, Institute for Virology and Immunobiology, University of Wuerzburg, D‑97080 Wuerzburg, Germany, Department of Otorhinolaryngology, Head and Neck Surgery ‘Otto Körner’, University Medical Center Rostock, D‑18057 Rostock, Germany
Published online on: May 2, 2018 https://doi.org/10.3892/ijmm.2018.3653
Pages: 1116-1124

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Abstract

In patients with chronic rhinosinusitis with nasal polyps (CRSwNP), a relative accumulation of cluster of differentiation (CD)8+ T cells over CD4+ T cells occurs in nasal polyps compared with the peripheral blood. Nasal CD8+ T cells and CD4+ T cells predominantly present an effector memory phenotype. Immunological studies have reported that memory T cells recirculate from the tissues to the peripheral blood and a high percentage of these T cells persist within the tissue. The aim of the present study was to characterize CD69+ sphingosine‑1‑phosphate receptor 1 (S1PR1)‑ tissue resident memory T cells (Trm) in the polyps of patients with CRSwNP. Tissue and blood samples were collected from 10 patients undergoing nasal sinus surgery. Expression of specific extra‑ and intracellular molecules were analyzed using multicolor flow cytometry. A significantly higher level of CD8+ T cells than CD4+ T cells was present in nasal polyps, while significantly more CD4+ T cells than CD8+ T cells were detected in the peripheral blood of patients with CRSwNP. The frequency of CD69+ T cells was significantly higher in CD8+ and CD4+ T cells in nasal polyps compared with the peripheral blood. The frequency of CD69+ S1PR1‑ Trm was also significantly higher in CD4+ and CD8+ T cells from nasal polyps compared with the peripheral blood. Within polyps, the frequency of CD69+ S1PR1‑ Trm was again significantly higher in CD8+ compared with CD4+ T cells. In summary, a significantly higher frequency of CD69+ S1PR1‑ T cells was observed in the nasal polyps compared with the peripheral blood in patients with CRSwNP. The results of the present study suggest that local regulation of the immune response occurs within nasal polyps. As such, Trm should be considered a potential stimulus in the pathogenesis of nasal polyps. However, the role of Trm in nasal polyps as a pathogenic trigger of the local inflammatory reaction requires further investigation.

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