Role of TGF‑β1 expressed in bone marrow‑derived mesenchymal stem cells in promoting bone formation in a rabbit femoral defect model

Sun,Bing‑Yin; Zhao,Bao‑Xiang; Zhu,Jie‑Ying; Sun,Zheng‑Ping; Shi,Yong‑An; Huang,Feng

doi:10.3892/ijmm.2018.3692

Role of TGF‑β1 expressed in bone marrow‑derived mesenchymal stem cells in promoting bone formation in a rabbit femoral defect model

Authors:
- Bing‑Yin Sun
- Bao‑Xiang Zhao
- Jie‑Ying Zhu
- Zheng‑Ping Sun
- Yong‑An Shi
- Feng Huang
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Affiliations: Department of Orthopedics, Shunde Hospital of Guangzhou University of Chinese Medicine, Foshan, Guangdong 528200, P.R. China, Department of Orthopedics, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China, Department of Substance Dependence, The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou, Guangdong 510370, P.R. China, Physical Examination Center, The Second Traditional Chinese Medicine Hospital of Guangdong Provence, Guangzhou, Guangdong 510095, P.R. China, Department of Microsurgery II, Wendeng Hospital of Traditional Chinese of Orthopedics and Traumatology of Shandong, Wendeng, Shandong 264400, P.R. China, Department of Orthopedics, The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China
Published online on: May 17, 2018 https://doi.org/10.3892/ijmm.2018.3692
Pages: 897-904
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bone defects represent a major clinical and socioeconomic problem without suitable treatment options. Previous studies have shown that transforming growth factor β1 (TGF‑β1) is important in the development of various diseases. The present study aimed to investigate the therapeutic potential of rabbit bone marrow‑derived mesenchymal stem cells (BMSCs) expressing TGF‑β1 in the treatment of rabbit femoral defects. First, rabbit BMSCs were identified and cultured. TGF‑β1 was then stably overexpressed in the rabbit BMSCs by lentivirus transfection, which was expressed at a high level in the femoral defects treated with TGF‑β1‑overexpressing BMSCs, compared with PBS‑treated controls. In addition, the TGF‑β1‑overexpressing BMSCs promoted new bone formation in the rabbit femoral defect model, and increased the expression of bone‑related markers at week 2 and week 6. Therefore, the study demonstrated that BMSCs overexpressing TGF‑β1 may provide a novel therapeutic option for femoral defects.

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