Characteristics of circular RNA expression in lung tissues from mice with hypoxia‑induced pulmonary hypertension

Wang,Jian; Zhu,Meng‑Chan; Kalionis,Bill; Wu,Jun‑Zhen; Wang,Lin‑Lin; Ge,Hai‑Yan; Chen,Cui‑Cui; Tang,Xiao‑Dan; Song,Yuan‑Lin; He,Hong; Xia,Shi‑Jin

doi:10.3892/ijmm.2018.3740

Characteristics of circular RNA expression in lung tissues from mice with hypoxia‑induced pulmonary hypertension

Authors:
- Jian Wang
- Meng‑Chan Zhu
- Bill Kalionis
- Jun‑Zhen Wu
- Lin‑Lin Wang
- Hai‑Yan Ge
- Cui‑Cui Chen
- Xiao‑Dan Tang
- Yuan‑Lin Song
- Hong He
- Shi‑Jin Xia
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Affiliations: Department of Geriatrics, Shanghai Institute of Geriatrics, Huadong Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200030, P.R. China, Pregnancy Research Centre, Department of Maternal‑Fetal Medicine, Royal Women's Hospital, Parkville, Victoria 3052, Australia, Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200030, P.R. China, Department of Pulmonary Medicine, Huadong Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Anesthesiology, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China
Published online on: June 21, 2018 https://doi.org/10.3892/ijmm.2018.3740
Pages: 1353-1366
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pulmonary hypertension (PH) is a life‑threatening lung disease, characterized by an increase in pulmonary arterial pressure caused by vasoconstriction and vascular remodeling. The pathogenesis of PH is not fully understood, and there is a lack of potential biomarkers for the diagnosis and treatment of patients with PH. Non‑coding RNAs with a characteristic covalently closed loop structure, termed circular RNAs (circRNAs), are present in a number of pulmonary diseases. To the best of our knowledge, the present study is the first to use microarray analysis to determine the expression profile of circRNAs in lung tissues from mice with hypoxia‑induced PH. In total, 23 significantly upregulated and 41 significantly downregulated circRNAs were identified. Of these, 12 differentially expressed circRNAs were selected for further validation using reverse transcription‑quantitative polymerase chain reaction. Putative microRNAs (miRNAs) that bind to the dysregulated circRNAs were predicted. Subsequently, bioinformatics tools were used to construct circRNA‑miRNA‑mRNA networks for the two most promising circRNAs, namely mmu_circRNA_004592 and mmu_circRNA_018351. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses of target genes of the dysregulated circRNAs revealed that these dysregulated circRNAs may serve an important role in the pathogenesis of hypoxia‑induced PH. Therefore, these dysregulated circRNAs are candidate diagnostic biomarkers and potential therapeutic targets for PH.

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