SFMBT2 positively regulates SOX9 and chondrocyte proliferation

  • Authors:
    • Safdar Hussain
    • Mengyao Sun
    • Yuanxu Guo
    • Nosheen Mushtaq
    • Yitong Zhao
    • Ying Yuan
    • Nazim Hussain
    • Ezra Osoro
    • Abubakar Suleiman
    • Muhammad Sadiq
    • Fujun Zhang
    • Yan Han
    • Jian Sun
    • Shemin Lu
  • View Affiliations

  • Published online on: September 25, 2018     https://doi.org/10.3892/ijmm.2018.3894
  • Pages: 3503-3512
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

SRY‑box 9 (SOX9) is the master regulator of the chondrocyte phenotype, which is essential for differentiating chondrogenic mesenchymal condensations into chondrocytes, and is involved in regulating every stage of chondrocyte differentiation. SOX9 deletion in chondrocytes at the late stages of cartilage development results in decreased chondrocyte proliferation; inhibited expression of cartilage matrix genes, including Indian hedgehog and the downstream parathyroid hormone‑related protein; and premature conversion of proliferating chondrocytes into hypertrophic chondrocytes, which mineralize their matrix prematurely. Therefore, SOX9 is considered vital for the majority of phases of chondrocyte lineage, from early condensations to the differentiation of proliferating chondrocytes, leading to chondrocyte hypertrophy. It has been reported that SOX9 expression is decreased in osteoarthritis (OA) cartilage. Regeneration or repair of cartilage degradation in OA remains a challenge. Previous studies have indicated that overexpression of SOX9 can promote cartilage repair and can be used as a potential therapeutic agent at the early stages of human OA. The present study identified Scm‑like with four malignant brain tumor domains 2 (SFMBT2) as a novel regulator of SOX9 expression in human chondrocytes. Our previous study revealed that SFMBT2 is negatively regulated in OA cartilage, and decreased levels of SFMBT2 contribute to the catabolic phenotype of chondrocytes. The present study detected increased expression levels of SFMBT2 in early cartilage development and during the early phases of chondrogenesis. Overexpression of SFMBT2 in C28/I2 cells upregulated SOX9 expression in a dose‑dependent manner. Furthermore, SFMBT2 positively regulated C28/I2 cell proliferation and restored the decreased levels of SOX9 in chondrocytes following tumor necrosis factor‑α treatment. Additional studies may reveal novel insights into the molecular mechanism involved and the potential role of SFMBT2 in cartilage repair and OA management.
View Figures
View References

Related Articles

Journal Cover

December-2018
Volume 42 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hussain S, Sun M, Guo Y, Mushtaq N, Zhao Y, Yuan Y, Hussain N, Osoro E, Suleiman A, Sadiq M, Sadiq M, et al: SFMBT2 positively regulates SOX9 and chondrocyte proliferation. Int J Mol Med 42: 3503-3512, 2018
APA
Hussain, S., Sun, M., Guo, Y., Mushtaq, N., Zhao, Y., Yuan, Y. ... Lu, S. (2018). SFMBT2 positively regulates SOX9 and chondrocyte proliferation. International Journal of Molecular Medicine, 42, 3503-3512. https://doi.org/10.3892/ijmm.2018.3894
MLA
Hussain, S., Sun, M., Guo, Y., Mushtaq, N., Zhao, Y., Yuan, Y., Hussain, N., Osoro, E., Suleiman, A., Sadiq, M., Zhang, F., Han, Y., Sun, J., Lu, S."SFMBT2 positively regulates SOX9 and chondrocyte proliferation". International Journal of Molecular Medicine 42.6 (2018): 3503-3512.
Chicago
Hussain, S., Sun, M., Guo, Y., Mushtaq, N., Zhao, Y., Yuan, Y., Hussain, N., Osoro, E., Suleiman, A., Sadiq, M., Zhang, F., Han, Y., Sun, J., Lu, S."SFMBT2 positively regulates SOX9 and chondrocyte proliferation". International Journal of Molecular Medicine 42, no. 6 (2018): 3503-3512. https://doi.org/10.3892/ijmm.2018.3894