Astragaloside IV promotes the proliferation and migration of osteoblast-like cells through the hedgehog signaling pathway

Guo,Li‑Hua; Cao,Yu; Zhuang,Run‑Tao; Han,Yan; Li,Jun

doi:10.3892/ijmm.2018.4013

Astragaloside IV promotes the proliferation and migration of osteoblast-like cells through the hedgehog signaling pathway

Authors:
- Li‑Hua Guo
- Yu Cao
- Run‑Tao Zhuang
- Yan Han
- Jun Li
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Affiliations: Department of Dental Implant Center, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing 100050, P.R. China, Department of Integrated Emergency Dental Care, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing 100050, P.R. China, Department of Stomatology, Beijing Jiaotong University Community Health Center, Beijing 100044, P.R. China, Department of Stomatology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091, P.R. China
Published online on: December 3, 2018 https://doi.org/10.3892/ijmm.2018.4013
Pages: 830-838
Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the effects of astragaloside IV on osteoblast‑like cell proliferation and migration, in addition to the underlying signaling pathway. In order to observe the effect on proliferation, a Cell Counting Kit‑8 assay and flow cytometry were used. To detect cell migration ability, cell scratch and Transwell cell migration assays were performed. The RNA and protein expression levels of hedgehog signaling molecules, including Sonic hedgehog (SHH) and GLI family zinc finger 1 (GLI1), were examined by reverse transcription‑quantitative polymerase chain reaction and western blot analyses. To inhibit the hedgehog signaling pathway, cyclopamine was used. Astragaloside IV, at a dosage of 1x10‑2 µg/ml in MG‑63 cells and 1x10‑3 µg/ml in U‑2OS cells, resulted in the enhanced proliferation and migration of cells, and the gene expression levels of the SHH and GLI1 were significantly increased. The combination of astragaloside IV and cyclopamine reduced MG‑63 and U‑2OS cell proliferation and migration, and inhibited the gene expression of SHH and GLI1. Astragaloside IV enhanced the proliferation and migration of human osteoblast‑like cells through activating the hedgehog signaling pathway. The results of the present study provide a rational for the mechanistic link in astragaloside IV promoting the proliferation and migration of osteoblasts via the hedgehog signaling pathway.

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