MicroRNA‑223 promotes osteoblast differentiation of MC3T3‑E1 cells by targeting histone deacetylase 2

  • Authors:
    • Jianming Chen
    • Guisong He
    • Yi Wang
    • Daozhang Cai
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  • Published online on: December 31, 2018     https://doi.org/10.3892/ijmm.2018.4042
  • Pages: 1513-1521
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Abstract

MicroRNAs (miRNAs) have emerged as pivotal regulators in various physiological and pathological processes at the post‑transcriptional level, and may serve important roles in osteogenic differentiation. However, their roles and functions are not fully understood. In the present study, miR‑223‑5p was identified as a modulator of osteoblastic differentiation in MC3T3‑E1 cells. Reverse transcription‑quantitative polymerase chain reaction and western blotting demonstrated that miR‑223‑5p was significantly upregulated in MC3T3‑E1 cells following the induction of osteoblast differentiation. Overexpression of miR‑223‑5p promoted osteogenic differentiation both in vitro and vivo. Expression of histone deacetylase 2 (HDAC2), which acts as a negative regulator of osteogenesis, was regulated by miR‑223‑5p. Collectively, the results of the present study revealed a novel miR‑223‑5p/HDAC2 axis that regulates osteoblast differentiation, and may serve as a potential target for enhancing bone formation in vivo.
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March-2019
Volume 43 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chen J, He G, Wang Y and Cai D: MicroRNA‑223 promotes osteoblast differentiation of MC3T3‑E1 cells by targeting histone deacetylase 2. Int J Mol Med 43: 1513-1521, 2019
APA
Chen, J., He, G., Wang, Y., & Cai, D. (2019). MicroRNA‑223 promotes osteoblast differentiation of MC3T3‑E1 cells by targeting histone deacetylase 2. International Journal of Molecular Medicine, 43, 1513-1521. https://doi.org/10.3892/ijmm.2018.4042
MLA
Chen, J., He, G., Wang, Y., Cai, D."MicroRNA‑223 promotes osteoblast differentiation of MC3T3‑E1 cells by targeting histone deacetylase 2". International Journal of Molecular Medicine 43.3 (2019): 1513-1521.
Chicago
Chen, J., He, G., Wang, Y., Cai, D."MicroRNA‑223 promotes osteoblast differentiation of MC3T3‑E1 cells by targeting histone deacetylase 2". International Journal of Molecular Medicine 43, no. 3 (2019): 1513-1521. https://doi.org/10.3892/ijmm.2018.4042