Circular RNA expression profiles in neonatal rats following hypoxic-ischemic brain damage

Jiang,Li; Li,Huijuan; Fan,Zhongmin; Zhao,Ruibin; Xia,Zhengkun

doi:10.3892/ijmm.2019.4111

Circular RNA expression profiles in neonatal rats following hypoxic-ischemic brain damage

Authors:
- Li Jiang
- Huijuan Li
- Zhongmin Fan
- Ruibin Zhao
- Zhengkun Xia
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Affiliations: Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210097, P.R. China, Department of Pediatrics, The BenQ Hospital, Nanjing Medical University, Nanjing, Jiangsu 210019, P.R. China, Department of Pediatrics, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225000, P.R. China, Department of Pediatrics, Jinling Hospital, Nanjing University, Nanjing, Jiangsu 210093, P.R. China
Published online on: February 26, 2019 https://doi.org/10.3892/ijmm.2019.4111
Pages: 1699-1708
Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNAs (circRNAs) have been studied in a number of diseases. However, the roles of circRNAs in hypoxic‑ischemic brain damage (HIBD) remains unknown. In the present study, high throughput sequencing was used to profile altered circRNAs in HIBD rats. A total of 66 circRNAs were identified to be differentially expressed (fold‑change >2 and P‑value <0.05) in HIBD rats compared with the control group, including 20 upregulated and 46 downregulated circRNAs. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that numerous mRNAs transcribed from the host genes of altered circRNAs were involved in brain damage and neural regeneration. The interaction of circRNA/microRNA was predicted based on TargetScan and miRanda. The results of this study demonstrated an altered circRNA expression pattern in HIBD rats and suggests important roles in HIBD physiological and pathological processes. These findings suggest a novel focus for future studies investigating the molecular mechanism underlying HIBD and possibilities for the treatment of HIBD through modulating circRNAs.

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