Anti‑inflammatory effects of 6‑formyl umbelliferone via the NF‑κB and ERK/MAPK pathway on LPS‑stimulated RAW 264.7 cells

Kim,Sang‑Bo; Kang,Min‑Jae; Kang,Chang‑Won; Kim,Nan‑Hee; Choi,Hyung   Wook; Jung,Hyun   Ah; Choi,Jae   Sue; Kim,Gun‑Do

doi:10.3892/ijmm.2019.4078

Anti‑inflammatory effects of 6‑formyl umbelliferone via the NF‑κB and ERK/MAPK pathway on LPS‑stimulated RAW 264.7 cells

Authors:
- Sang‑Bo Kim
- Min‑Jae Kang
- Chang‑Won Kang
- Nan‑Hee Kim
- Hyung Wook Choi
- Hyun Ah Jung
- Jae Sue Choi
- Gun‑Do Kim
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Affiliations: Department of Microbiology, College of Natural Sciences, Pukyong National University, Busan 48513, Republic of Korea, Australian Cancer Research Foundation, Department of Cancer Biology and Therapeutics, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia, Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju 54896, Republic of Korea, Department of Food and Life Science, Pukyong National University, Busan 48513, Republic of Korea
Published online on: January 25, 2019 https://doi.org/10.3892/ijmm.2019.4078
Pages: 1859-1865

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Abstract

Inhibition of over‑activated inflammation has been demonstrated as one of the most efficient strategies for treating inflammatory diseases. In the present study, 6‑formyl umbelliferone (6FU) was used to evaluate its anti‑inflammatory effects on lipopolysaccharide (LPS)‑stimulated RAW 264.7 macrophages. 6FU inhibited chronic inflammatory processes, including increasing nitric oxide levels, and the expression of pro‑inflammatory genes and producing cytokines was investigated by a nitrite assay and reverse transcription‑polymerase chain reaction, respectively. Nitric oxide and pro‑inflammatory cytokines, including tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6 were decreased by treatment with 6FU, without cell cytotoxicity in LPS‑stimulated RAW 264.7 cells, which was measured by a WST‑1 assay. In the western blot analysis, the expression levels of phosphorylated extracellular signal‑regulated kinase (ERK)1/2 was downregulated in 6FU‑treated cells. Furthermore, in the western blotting and immunofluorescence staining results, translocation activities of ERK1/2 and NF‑κB from the cytoplasm to the nucleus were suppressed, which may inhibit translation of numerous proteins associated with pro‑inflammation, including inducible nitric oxide synthase and cyclooxygenase‑2. Therefore, based on these results, it was suggested that 6FU may be a potential candidate for the development of agents against chronic inflammation.

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