PHOSPHORYLATION OF WILD-TYPE AND MUTANT PHENOTYPES OF P53 BY AN ASSOCIATED PROTEIN-KINASE

  • Authors:
    • A LORENZ
    • CPE HERRMANN
    • OG ISSINGER
    • M MONTENARH
  • View Affiliations

  • Published online on: October 1, 1992     https://doi.org/10.3892/ijo.1.5.571
  • Pages: 571-579
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Abstract

Immunopurified mutant mouse p53 from transformed cells is known to be tightly associated with a protein kinase which phosphorylates p53 in an in vitro kinase reaction. Wild-type p53 from a non-transformed cell line was not associated with a protein kinase whereas immunopurified wild-type p53 from a transformed cell line was tightly associated with a protein kinase which phosphorylated p53. In order to compare wild-type and mutant p53 in the same cellular environment both forms were cloned in a baculovirus expression system and in in vitro transcription/translation vectors and both p53 proteins were expressed in the two systems. Wild-type and mutant p53 from baculovirus infected insect cells were tightly associated with a protein kinase which phosphorylates p53. In contrast, immunopurified wild-type and mutant p53 from an in vitro transcription/translation reaction were not associated with a protein kinase but could be phosphorylated by added casein kinase II. Thus, in the present paper we demonstrate that the association of p53 with a protein kinase and the in vitro phosphorylation of p53 seems to depend on the surrounding cellular environment.

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October 1992
Volume 1 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
LORENZ A, HERRMANN C, ISSINGER O and MONTENARH M: PHOSPHORYLATION OF WILD-TYPE AND MUTANT PHENOTYPES OF P53 BY AN ASSOCIATED PROTEIN-KINASE. Int J Oncol 1: 571-579, 1992
APA
LORENZ, A., HERRMANN, C., ISSINGER, O., & MONTENARH, M. (1992). PHOSPHORYLATION OF WILD-TYPE AND MUTANT PHENOTYPES OF P53 BY AN ASSOCIATED PROTEIN-KINASE. International Journal of Oncology, 1, 571-579. https://doi.org/10.3892/ijo.1.5.571
MLA
LORENZ, A., HERRMANN, C., ISSINGER, O., MONTENARH, M."PHOSPHORYLATION OF WILD-TYPE AND MUTANT PHENOTYPES OF P53 BY AN ASSOCIATED PROTEIN-KINASE". International Journal of Oncology 1.5 (1992): 571-579.
Chicago
LORENZ, A., HERRMANN, C., ISSINGER, O., MONTENARH, M."PHOSPHORYLATION OF WILD-TYPE AND MUTANT PHENOTYPES OF P53 BY AN ASSOCIATED PROTEIN-KINASE". International Journal of Oncology 1, no. 5 (1992): 571-579. https://doi.org/10.3892/ijo.1.5.571