Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'-methoxyphenoxy)benzoate (DIME)

  • Authors:
    • K Buki
    • E Kirsten
    • P Bauer
    • C Vidair
    • A Kun
    • J Mendeleyev
    • E Kun
  • View Affiliations

  • Published online on: December 1, 1997     https://doi.org/10.3892/ijo.11.6.1247
  • Pages: 1247-1250
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Abstract

The GTPase activity of purified dimeric tubulin (alpha+beta) at 5 mu M was insensitive to methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME), in contrast to nocodazole which activated GTPase. Cellular motility of MDA-MB-231 (human mammary cancer) cells migrating through 12-mu m pores was inhibited by DIME similar to nocodazole in a drug concentration-and DIME structure-dependent manner. An increase of cytoplasmic ATPase activity of DIME-treated cells without a decrease in ATP contents of intact cells suggests that DIME may also influence additional as yet unidentified ATP-dependent system(s) probably also involved in cell motility. These results show that DIME not only arrests cells in M phase but also inhibits cell motility in interphase. However the cellular mode of action of DIME is different from the action of other toxic tubulin-targeted drugs, despite the fact that DIME in a concentration-dependent manner disrupts microtubule structures in intact cells.

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December 1997
Volume 11 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Buki K, Kirsten E, Bauer P, Vidair C, Kun A, Mendeleyev J and Kun E: Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'-methoxyphenoxy)benzoate (DIME). Int J Oncol 11: 1247-1250, 1997
APA
Buki, K., Kirsten, E., Bauer, P., Vidair, C., Kun, A., Mendeleyev, J., & Kun, E. (1997). Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'-methoxyphenoxy)benzoate (DIME). International Journal of Oncology, 11, 1247-1250. https://doi.org/10.3892/ijo.11.6.1247
MLA
Buki, K., Kirsten, E., Bauer, P., Vidair, C., Kun, A., Mendeleyev, J., Kun, E."Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'-methoxyphenoxy)benzoate (DIME)". International Journal of Oncology 11.6 (1997): 1247-1250.
Chicago
Buki, K., Kirsten, E., Bauer, P., Vidair, C., Kun, A., Mendeleyev, J., Kun, E."Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'-methoxyphenoxy)benzoate (DIME)". International Journal of Oncology 11, no. 6 (1997): 1247-1250. https://doi.org/10.3892/ijo.11.6.1247