Induction of antitumor effect on human esophageal carcinoma cells by the retroviral expression of granulocyte macrophage-colony stimulating factor gene.

  • Authors:
    • M Sugaya
    • M Tagawa
    • H Matsubara
    • Y Gunji
    • K Takenaga
    • T Maeda
    • Y Koide
    • T Asano
    • T Ochiai
    • K Isono
    • S Sakiyama
  • View Affiliations

  • Published online on: February 1, 1998     https://doi.org/10.3892/ijo.12.2.321
  • Pages: 321-325
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Abstract

We have examined antitumor effect of human esophageal carcinoma cells (T.Tn) which were retrovirally transduced to express mouse granulocyte macrophage-colony stimulating factor (mGM-CSF) gene. Nude mice inoculated with T.Tn cells secreting mGM-CSF developed small tumors but the tumors regressed spontaneously, although the proliferation in vitro of transduced cells was not different from that of wild-type cells. In contrast, the tumor of T.Tn cells transduced with human GM-CSF grew as that of wild-type cells, since murine GM-CSF receptors do not bind to human GM-CSF. Histological examination of the regressing tumor of mGM-CSF-producing T.Tn cells revealed predominant infiltration of inflammatory cells including macrophages. In addition, local injection of mGM-CSF-producing T.Tn cells into the established wild-type tumors significantly induced the retardation of subsequent wild-type tumor growth, suggesting that T-cell independent local response plays a crucial role in destroying tumor cells.

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Feb 1998
Volume 12 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sugaya M, Tagawa M, Matsubara H, Gunji Y, Takenaga K, Maeda T, Koide Y, Asano T, Ochiai T, Isono K, Isono K, et al: Induction of antitumor effect on human esophageal carcinoma cells by the retroviral expression of granulocyte macrophage-colony stimulating factor gene.. Int J Oncol 12: 321-325, 1998.
APA
Sugaya, M., Tagawa, M., Matsubara, H., Gunji, Y., Takenaga, K., Maeda, T. ... Sakiyama, S. (1998). Induction of antitumor effect on human esophageal carcinoma cells by the retroviral expression of granulocyte macrophage-colony stimulating factor gene.. International Journal of Oncology, 12, 321-325. https://doi.org/10.3892/ijo.12.2.321
MLA
Sugaya, M., Tagawa, M., Matsubara, H., Gunji, Y., Takenaga, K., Maeda, T., Koide, Y., Asano, T., Ochiai, T., Isono, K., Sakiyama, S."Induction of antitumor effect on human esophageal carcinoma cells by the retroviral expression of granulocyte macrophage-colony stimulating factor gene.". International Journal of Oncology 12.2 (1998): 321-325.
Chicago
Sugaya, M., Tagawa, M., Matsubara, H., Gunji, Y., Takenaga, K., Maeda, T., Koide, Y., Asano, T., Ochiai, T., Isono, K., Sakiyama, S."Induction of antitumor effect on human esophageal carcinoma cells by the retroviral expression of granulocyte macrophage-colony stimulating factor gene.". International Journal of Oncology 12, no. 2 (1998): 321-325. https://doi.org/10.3892/ijo.12.2.321