p53 modulation of Fas/Apo-1 mediated apoptosis in a human renal cell carcinoma cell line.

  • Authors:
    • H Miyake
    • I Hara
    • K Gohji
    • S Arakawa
    • S Kamidono
  • View Affiliations

  • Published online on: February 1, 1998     https://doi.org/10.3892/ijo.12.2.469
  • Pages: 469-542
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The mutant p53 gene was transfected into ACHN, a wild-type p53-containing human renal cell carcinoma (RCC) cell line. The colony forming efficiency in soft agar in the mutant-type p53-transfected cell line (ACHN/MP) was significantly higher than that in the vector-only transfected control cell line (ACHN/C). The anti-Fas monoclonal antibody (CH11) induced apoptosis in the ACHN/C cells in a dose-dependent manner, whereas the effect of CH11 on the ACHN/ MP cells was markedly suppressed. In addition, the cytotoxic effect of CH11 on the ACHN/MP cells was augmented by the pretreatment with interferon- , but the corresponding effect on ACHN/C cells was not. These findings suggest that Fas-mediated therapy could be a novel approach to RCC, if interferon- treatment is added according to the p53 gene status.

Related Articles

Journal Cover

Feb 1998
Volume 12 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Miyake H, Hara I, Gohji K, Arakawa S and Kamidono S: p53 modulation of Fas/Apo-1 mediated apoptosis in a human renal cell carcinoma cell line.. Int J Oncol 12: 469-542, 1998.
APA
Miyake, H., Hara, I., Gohji, K., Arakawa, S., & Kamidono, S. (1998). p53 modulation of Fas/Apo-1 mediated apoptosis in a human renal cell carcinoma cell line.. International Journal of Oncology, 12, 469-542. https://doi.org/10.3892/ijo.12.2.469
MLA
Miyake, H., Hara, I., Gohji, K., Arakawa, S., Kamidono, S."p53 modulation of Fas/Apo-1 mediated apoptosis in a human renal cell carcinoma cell line.". International Journal of Oncology 12.2 (1998): 469-542.
Chicago
Miyake, H., Hara, I., Gohji, K., Arakawa, S., Kamidono, S."p53 modulation of Fas/Apo-1 mediated apoptosis in a human renal cell carcinoma cell line.". International Journal of Oncology 12, no. 2 (1998): 469-542. https://doi.org/10.3892/ijo.12.2.469