A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane
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- Published online on: September 1, 2003 https://doi.org/10.3892/ijo.23.3.745
- Pages: 745-750
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Abstract
Hypoxia is an important regulator of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) expression. Using Dunning rat prostate tumour cells (MatLyLu) we examined the induction of HIF-1α and VEGF by hypoxia and if the induction of these proteins would lead to angiogenesis in the chick chorioallantoic membrane (CAM). MatLyLu cells were exposed to 1% O2, in 5.25% CO2 and 94.75% N2, or treated with 100 mM CoCl2 to simulate hypoxia. Conditioned medium was analyzed using Western blots for HIF-1α or VEGF. VEGF levels were quantified using ELISA. Hypoxia significantly increased both HIF-1α and VEGF protein production. MatLyLu cells (1x106 viable cells in 50 µl of medium) grown under normoxic conditions induced in angiogenesis in the CAM, evaluated by the formation of a spoke wheel, and in cross sections by the number of blood vessels, following 5-day culture. This response was significantly increased using CoCl2-treated cells. Culture medium alone with or without CoCl2 was not angiogenic. These results provide direct evidence for the role of hypoxia in the induction of HIF-1α and VEGF which act as key angiogenic signals.