Immunization with live HPV-16-transformed mouse cells expressing the herpes simplex thymidine kinase and either GM-CSF or IL-2

  • Authors:
    • Pavel Jinoch
    • Roman Zak
    • Olga Janousková
    • David Kunke
    • Simon Rittich
    • Martina Duskova
    • Eva Sobotkova
    • Iuri Marinov
    • Monika Andelova
    • Michal Smahel
    • Vladimir Vonka
  • View Affiliations

  • Published online on: September 1, 2003     https://doi.org/10.3892/ijo.23.3.775
  • Pages: 775-783
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

From mouse (C57BL/6) HPV-16 transformed cells denoted MK16/1/IIIABC (MK16) a cellular thymidine kinase deficient (cTK-) cell line was isolated. These cTK- cells were transduced by bicistronic recombinant adeno-associated viruses (rAAV) carrying the herpes simplex virus thymidine kinase gene and the gene for either the mouse granulocyte-macrophage colony stimulating factor (GM-CSF) or mouse interleukin-2 (IL-2). Transduced cells were highly sensitive to minute amounts of ganciclovir (GCV) and synthesized moderate amounts of the respective cytokines. A number of cell clones were tested for the cytokine production. The two best producer cell lines, the GM-CSF-producing cells denoted B9 and the IL-2-producing cells denoted 181, were selected for further experiments. Neither B9 nor 181 cells were tumorigenic in syngeneic animals. As inducers of antitumour immunity against challenge with MK16 cells, B9 cells proved superior to the 181 cells. GCV treatment did not markedly influence the level of immunity induced.

Related Articles

Journal Cover

September 2003
Volume 23 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Jinoch P, Zak R, Janousková O, Kunke D, Rittich S, Duskova M, Sobotkova E, Marinov I, Andelova M, Smahel M, Smahel M, et al: Immunization with live HPV-16-transformed mouse cells expressing the herpes simplex thymidine kinase and either GM-CSF or IL-2. Int J Oncol 23: 775-783, 2003
APA
Jinoch, P., Zak, R., Janousková, O., Kunke, D., Rittich, S., Duskova, M. ... Vonka, V. (2003). Immunization with live HPV-16-transformed mouse cells expressing the herpes simplex thymidine kinase and either GM-CSF or IL-2. International Journal of Oncology, 23, 775-783. https://doi.org/10.3892/ijo.23.3.775
MLA
Jinoch, P., Zak, R., Janousková, O., Kunke, D., Rittich, S., Duskova, M., Sobotkova, E., Marinov, I., Andelova, M., Smahel, M., Vonka, V."Immunization with live HPV-16-transformed mouse cells expressing the herpes simplex thymidine kinase and either GM-CSF or IL-2". International Journal of Oncology 23.3 (2003): 775-783.
Chicago
Jinoch, P., Zak, R., Janousková, O., Kunke, D., Rittich, S., Duskova, M., Sobotkova, E., Marinov, I., Andelova, M., Smahel, M., Vonka, V."Immunization with live HPV-16-transformed mouse cells expressing the herpes simplex thymidine kinase and either GM-CSF or IL-2". International Journal of Oncology 23, no. 3 (2003): 775-783. https://doi.org/10.3892/ijo.23.3.775