Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: Analysis of the ability to support expansion of cord blood hematopoietic progenitor cells

  • Authors:
    • Keisuke Nishioka
    • Yoshihiro Fujimori
    • Tomoko Hashimoto-Tamaoki
    • Shunro Kai
    • Huiying Qiu
    • Naoya Kobayashi
    • Noriaki Tanaka
    • Karen A. Westerman
    • Philippe Leboulch
    • Hiroshi Hara
  • View Affiliations

  • Published online on: October 1, 2003     https://doi.org/10.3892/ijo.23.4.925
  • Pages: 925-932
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Abstract

Human marrow-derived mesenchymal stem cells (MSC), which have the potential to differentiate into mesenchymal tissues, such as bone, cartilage, adipose and bone marrow stroma, were transduced with a retroviral vector carrying the simian virus 40 large T antigen, hygromycin-resistant gene and herpes simplex virus thymidine kinase gene, that can be excised by Cre/loxP site-specific recombination. This resulted in establishment of an MSC cell line, HMSC-1, which retained original surface characteristics and differentiation potential, and exhibited a higher proliferative capacity than parental cells. HMSC-1 expressed mRNAs of BMP-4, Jagged-1, and SCF that are known to promote hematopoiesis. Human CB CD34+ hematopoietic progenitor cells (HPC) cultured on a layer of HMSC-1 cells showed high expansion of CD34+CD38− immature HPC, capable of reconstituting human hematopoiesis in non-obese diabetic/severe combined immunodeficient disease (NOD/SCID) mice. This cell line may be of value for developing strategies for ex vivo expansion of human HPC.

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October 2003
Volume 23 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Nishioka K, Fujimori Y, Hashimoto-Tamaoki T, Kai S, Qiu H, Kobayashi N, Tanaka N, Westerman KA, Leboulch P, Hara H, Hara H, et al: Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: Analysis of the ability to support expansion of cord blood hematopoietic progenitor cells. Int J Oncol 23: 925-932, 2003.
APA
Nishioka, K., Fujimori, Y., Hashimoto-Tamaoki, T., Kai, S., Qiu, H., Kobayashi, N. ... Hara, H. (2003). Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: Analysis of the ability to support expansion of cord blood hematopoietic progenitor cells. International Journal of Oncology, 23, 925-932. https://doi.org/10.3892/ijo.23.4.925
MLA
Nishioka, K., Fujimori, Y., Hashimoto-Tamaoki, T., Kai, S., Qiu, H., Kobayashi, N., Tanaka, N., Westerman, K. A., Leboulch, P., Hara, H."Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: Analysis of the ability to support expansion of cord blood hematopoietic progenitor cells". International Journal of Oncology 23.4 (2003): 925-932.
Chicago
Nishioka, K., Fujimori, Y., Hashimoto-Tamaoki, T., Kai, S., Qiu, H., Kobayashi, N., Tanaka, N., Westerman, K. A., Leboulch, P., Hara, H."Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: Analysis of the ability to support expansion of cord blood hematopoietic progenitor cells". International Journal of Oncology 23, no. 4 (2003): 925-932. https://doi.org/10.3892/ijo.23.4.925