Exogenous expression of H-cadherin in CHO cells regulates contact inhibition of cell growth by inducing p21 expression

  • Authors:
    • Yun Zhong
    • Lluis Lopez-Barcons
    • Missak Haigentz
    • Yi-He Ling
    • Roman Perez-Soler
  • View Affiliations

  • Published online on: June 1, 2004     https://doi.org/10.3892/ijo.24.6.1573
  • Pages: 1573-1579
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Abstract

The impact of the cadherins in human cancers is becoming better understood. However, few studies have directly tested the hypothesis that H-cadherin, a tailless cadherin, is actually a tumor suppressor, and no published studies have addressed the question of how H-cadherin suppresses cellular transformation. We report here the influence that exogenous expression of H-cadherin imposes on growth, morphology, clonogenicity and tumorigenicity of Chinese hamster ovarian (CHO) cells. H-cadherin expression in CHO cells resulted in tighter adhesion of multicellular aggregates and reduced cell proliferation. In addition to enhancement of cell-cell adhesion, exogenous H-cadherin expression also inhibited cell proliferation and the ability to form colonies in soft agar. Furthermore, expression of H-cadherin in CHO cells led to complete suppression of subcutaneous tumor growth in nude mice. Seeding the H-cadherin expressing CHO cells on culture plates coated with recombinant H-cadherin amino-terminal fragments resulted in inhibition of cell proliferation that was accompanied by increased expression of the cdk inhibitor p21. These results support the role of H-cadherin as a tumor suppressor participating in contact inhibition of cell growth, possibly by inducing p21 expression.

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June 2004
Volume 24 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zhong Y, Lopez-Barcons L, Haigentz M, Ling Y and Perez-Soler R: Exogenous expression of H-cadherin in CHO cells regulates contact inhibition of cell growth by inducing p21 expression. Int J Oncol 24: 1573-1579, 2004.
APA
Zhong, Y., Lopez-Barcons, L., Haigentz, M., Ling, Y., & Perez-Soler, R. (2004). Exogenous expression of H-cadherin in CHO cells regulates contact inhibition of cell growth by inducing p21 expression. International Journal of Oncology, 24, 1573-1579. https://doi.org/10.3892/ijo.24.6.1573
MLA
Zhong, Y., Lopez-Barcons, L., Haigentz, M., Ling, Y., Perez-Soler, R."Exogenous expression of H-cadherin in CHO cells regulates contact inhibition of cell growth by inducing p21 expression". International Journal of Oncology 24.6 (2004): 1573-1579.
Chicago
Zhong, Y., Lopez-Barcons, L., Haigentz, M., Ling, Y., Perez-Soler, R."Exogenous expression of H-cadherin in CHO cells regulates contact inhibition of cell growth by inducing p21 expression". International Journal of Oncology 24, no. 6 (2004): 1573-1579. https://doi.org/10.3892/ijo.24.6.1573