Radioresistance is associated to increased Hsp70 content in human glioblastoma cell lines

  • Authors:
    • Adriana Brondani Da Rocha
    • Andrea Regner
    • Ivana Grivicich
    • Daniel Pretto Schunemann
    • Celito Diel
    • Giovana Kovaleski
    • Caroline Brunetto De Farias
    • Edlaine Mondadori
    • Leonardo Almeida
    • Aroldo Braga Filho
    • Gilberto Schwartsmann
  • View Affiliations

  • Published online on: September 1, 2004     https://doi.org/10.3892/ijo.25.3.777
  • Pages: 777-785
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Abstract

Radiation therapy is routinely prescribed for high-grade malignant gliomas. However, the efficacy of this therapeutic modality is often limited by the occurrence of radioresistance, reflected as a diminished susceptibility of the irradiated cells to undergo apoptosis. Heat-shock proteins (Hsps) synthesis can be increased by cellular insults, such as radiation-induced damage. Inducible Hsp70 has been suggested to have multiple roles in cytoprotection against apoptosis. Accordingly, high levels of Hsp70 prevent stress-induced apoptosis. In the present study, we investigated whether the content of Hsp70 is associated to glioblastoma cell radioresistance. To this end, the U-87MG, U-251MG and MO59J human glioblastoma cell lines were irradiated at 2, 5 and 10 Gy and their relative radioresistance and Hsp70 were determined. Following 5 Gy irradiation, in MO59J and U-251MG a significant decrease in colony formation was found, whereas the U-87MG was relatively radioresistant. Three hours after the irradiation (at 5 Gy) Hsp70 contents increased 110% in the U-87 MG cells, but did not significantly change in the U-251MG and MO59J cells. Thus, our results suggest that Hsp70 protection against radiation-induced apoptosis might underlie glioblastoma radioresistance.

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September 2004
Volume 25 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Brondani Da Rocha A, Regner A, Grivicich I, Pretto Schunemann D, Diel C, Kovaleski G, Brunetto De Farias C, Mondadori E, Almeida L, Braga Filho A, Braga Filho A, et al: Radioresistance is associated to increased Hsp70 content in human glioblastoma cell lines. Int J Oncol 25: 777-785, 2004.
APA
Brondani Da Rocha, A., Regner, A., Grivicich, I., Pretto Schunemann, D., Diel, C., Kovaleski, G. ... Schwartsmann, G. (2004). Radioresistance is associated to increased Hsp70 content in human glioblastoma cell lines. International Journal of Oncology, 25, 777-785. https://doi.org/10.3892/ijo.25.3.777
MLA
Brondani Da Rocha, A., Regner, A., Grivicich, I., Pretto Schunemann, D., Diel, C., Kovaleski, G., Brunetto De Farias, C., Mondadori, E., Almeida, L., Braga Filho, A., Schwartsmann, G."Radioresistance is associated to increased Hsp70 content in human glioblastoma cell lines". International Journal of Oncology 25.3 (2004): 777-785.
Chicago
Brondani Da Rocha, A., Regner, A., Grivicich, I., Pretto Schunemann, D., Diel, C., Kovaleski, G., Brunetto De Farias, C., Mondadori, E., Almeida, L., Braga Filho, A., Schwartsmann, G."Radioresistance is associated to increased Hsp70 content in human glioblastoma cell lines". International Journal of Oncology 25, no. 3 (2004): 777-785. https://doi.org/10.3892/ijo.25.3.777