Genes associated with human hepatocellular carcinoma cell chemosensitivity to 5-fluorouracil plus interferon-α combination chemotherapy

  • Authors:
    • Masaru Moriyama
    • Yujin Hoshida
    • Naoya Kato
    • Motoyuki Otsuka
    • Haruhiko Yoshida
    • Takao Kawabe
    • Masao Omata
  • View Affiliations

  • Published online on: November 1, 2004     https://doi.org/10.3892/ijo.25.5.1279
  • Pages: 1279-1287
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Abstract

Recently, combined chemotherapy with 5-fluorouracil (5-FU) and interferon (IFN)-α has been reported to show marked effects in patients with advanced hepatocellular carcinoma. We investigated the genes associated with susceptibility to this combination therapy. The gene expression profiles of eight human hepatocellular carcinoma cells (HepG2, Hep3B, Huh7, Huh6, PLC/PRF/5, HLE, HLF, and SK-Hep1) were evaluated using an oligonucleotide microarray that consisted of 3,800 genes. The 50% growth inhibitory concentration (GI50) values for 5-FU, IFN-α, and the combination of 5-FU plus IFN-α were determined by the MTT assay. We selected genes that were expressed differentially between the cells with increased susceptibility to the combination therapy and the remaining cells. Relevance networks of the gene expression patterns and GI50 values of the susceptible cells were constructed to find genes associated with susceptibility to the combination therapy. Of the eight cells tested, five showed increased susceptibility to 5-FU plus IFN-α compared with 5-FU treatment alone. Among the 3,800 genes, 25 were expressed differentially between susceptible cells and resistant cells. The relevance networks revealed that sensitivity to 5-FU plus IFN-α involved the expression of 27 independent genes, which included 10 genes that are commonly associated with sensitivity to 5-FU alone. We selected a set of genes to predict susceptibility to 5-FU plus IFN-α combination therapy. We also selected genes that play key roles in the synergistic effect of this combination therapy. These gene sets should prove useful in evaluations of the efficacy and underlying molecular mechanisms of this combination therapy.

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November 2004
Volume 25 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Moriyama M, Hoshida Y, Kato N, Otsuka M, Yoshida H, Kawabe T and Omata M: Genes associated with human hepatocellular carcinoma cell chemosensitivity to 5-fluorouracil plus interferon-α combination chemotherapy. Int J Oncol 25: 1279-1287, 2004
APA
Moriyama, M., Hoshida, Y., Kato, N., Otsuka, M., Yoshida, H., Kawabe, T., & Omata, M. (2004). Genes associated with human hepatocellular carcinoma cell chemosensitivity to 5-fluorouracil plus interferon-α combination chemotherapy. International Journal of Oncology, 25, 1279-1287. https://doi.org/10.3892/ijo.25.5.1279
MLA
Moriyama, M., Hoshida, Y., Kato, N., Otsuka, M., Yoshida, H., Kawabe, T., Omata, M."Genes associated with human hepatocellular carcinoma cell chemosensitivity to 5-fluorouracil plus interferon-α combination chemotherapy". International Journal of Oncology 25.5 (2004): 1279-1287.
Chicago
Moriyama, M., Hoshida, Y., Kato, N., Otsuka, M., Yoshida, H., Kawabe, T., Omata, M."Genes associated with human hepatocellular carcinoma cell chemosensitivity to 5-fluorouracil plus interferon-α combination chemotherapy". International Journal of Oncology 25, no. 5 (2004): 1279-1287. https://doi.org/10.3892/ijo.25.5.1279