Differentially expressed protein markers in human submandibular and sublingual secretions

  • Authors:
    • Shen Hu
    • Patricia Denny
    • Paul Denny
    • Yongming Xie
    • Joseph A. Loo
    • Lawrence E. Wolinsky
    • Yang Li
    • Jim McBride
    • Rachel R. Ogorzalek Loo
    • Mavash Navazesh
    • David T. Wong
  • View Affiliations

  • Published online on: November 1, 2004     https://doi.org/10.3892/ijo.25.5.1423
  • Pages: 1423-1430
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Abstract

Proteome analysis of secretions from individual salivary glands is important for understanding the health of the oral cavity and pathogenesis of certain diseases. However, cross-contamination of submandibular (SM) and sublingual (SL) glandular secretions can occur. The close anatomic relationship of the SM and SL ductal orifices can lead to such contamination. Additionally, these glands may share common ducts. To insure the purity of SM/SL secretions for proteomic analysis, it is important to develop unique biomarkers which could be used to verify the integrity of the individual glandular saliva. In this study, a proteomics approach based on mass spectrometry and gel electrophoresis techniques was utilized to identify and verify a set of proteins (cystatin C, calgranulin B and MUC5B mucin), which are differentially expressed in SM/SL secretions. SM/SL fluids were obtained from nine healthy subjects. Cystatin C was found to be an SM-selective protein as it was found in all SM fluids but not detected in two SL fluids. MUC5B mucin and calgranulin B, on the other hand, were found to be SL-selective proteins. All SL samples contained MUC5B mucin, whereas MUC5B mucin was not detected in four SM samples. Eight of the SL samples contained calgranulin B; however, calgranulin B was absent in eight SM samples. This set of protein markers, especially calgranulin B, can be used to determine the purity of SM/SL samples, and therefore identify potential individuals who do not exhibit cross-contaminated SM/SL secretions, an important requirement for subsequent proteome analysis of pure SM and SL secretions.

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November 2004
Volume 25 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hu S, Denny P, Denny P, Xie Y, Loo JA, Wolinsky LE, Li Y, McBride J, Ogorzalek Loo RR, Navazesh M, Navazesh M, et al: Differentially expressed protein markers in human submandibular and sublingual secretions. Int J Oncol 25: 1423-1430, 2004
APA
Hu, S., Denny, P., Denny, P., Xie, Y., Loo, J.A., Wolinsky, L.E. ... Wong, D.T. (2004). Differentially expressed protein markers in human submandibular and sublingual secretions. International Journal of Oncology, 25, 1423-1430. https://doi.org/10.3892/ijo.25.5.1423
MLA
Hu, S., Denny, P., Denny, P., Xie, Y., Loo, J. A., Wolinsky, L. E., Li, Y., McBride, J., Ogorzalek Loo, R. R., Navazesh, M., Wong, D. T."Differentially expressed protein markers in human submandibular and sublingual secretions". International Journal of Oncology 25.5 (2004): 1423-1430.
Chicago
Hu, S., Denny, P., Denny, P., Xie, Y., Loo, J. A., Wolinsky, L. E., Li, Y., McBride, J., Ogorzalek Loo, R. R., Navazesh, M., Wong, D. T."Differentially expressed protein markers in human submandibular and sublingual secretions". International Journal of Oncology 25, no. 5 (2004): 1423-1430. https://doi.org/10.3892/ijo.25.5.1423