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Prediction of chemosensitivity of colorectal cancer to 5-fluorouracil by gene expression profiling with cDNA microarrays

  • Authors:
    • Daisuke Shimizu
    • Takashi Ishikawa
    • Yasushi Ichikawa
    • Shinji Togo
    • Yoshihide Hayasizaki
    • Yasushi Okazaki
    • Peter. V Danenberg
    • Hiroshi Shimada

  • View Affiliations

  • Published online on: August 1, 2005     https://doi.org/10.3892/ijo.27.2.371
  • Pages: 371-376
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Abstract

5-Fluorouracil (5-FU) is the most widely used anticancer agent for gastrointestinal cancers. Because many tumors show primary resistance, it is clinically meaningful to predict tumor sensitivity to the drug before treatment. cDNA microarrays containing 21,168 clones were used to identify genes associated with sensitivity to 5-FU. Gene expression profiling of 3 colorectal cancer cell lines (DLD-1, HT-29 and NUGC-3) and the corresponding 5-FU-resistant sublines (DLD-1/FU, HT-29/FU and NUGC-3/5FU/L) showed 81 genes that were differentially expressed. The gene set thus identified successfully predicted the sensitivities of 5 other colorectal cancer cell lines and could also separate 5-FU resistant clinical samples from sensitive ones.

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August 2005
Volume 27 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Journal Metrics
  • Impact Factor: 5.2
  • Ranked Oncology
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  • CiteScore: 11.4
  • CiteScore Rank: Q1: #40/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 1.125
  • SCImago Journal Rank (SJR): 1.105
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Spandidos Publications style
Shimizu D, Ishikawa T, Ichikawa Y, Togo S, Hayasizaki Y, Okazaki Y, Danenberg PV and Shimada H: Prediction of chemosensitivity of colorectal cancer to 5-fluorouracil by gene expression profiling with cDNA microarrays. Int J Oncol 27: 371-376, 2005
APA
Shimizu, D., Ishikawa, T., Ichikawa, Y., Togo, S., Hayasizaki, Y., Okazaki, Y. ... Shimada, H. (2005). Prediction of chemosensitivity of colorectal cancer to 5-fluorouracil by gene expression profiling with cDNA microarrays. International Journal of Oncology, 27, 371-376. https://doi.org/10.3892/ijo.27.2.371
MLA
Shimizu, D., Ishikawa, T., Ichikawa, Y., Togo, S., Hayasizaki, Y., Okazaki, Y., Danenberg, P. V., Shimada, H."Prediction of chemosensitivity of colorectal cancer to 5-fluorouracil by gene expression profiling with cDNA microarrays". International Journal of Oncology 27.2 (2005): 371-376.
Chicago
Shimizu, D., Ishikawa, T., Ichikawa, Y., Togo, S., Hayasizaki, Y., Okazaki, Y., Danenberg, P. V., Shimada, H."Prediction of chemosensitivity of colorectal cancer to 5-fluorouracil by gene expression profiling with cDNA microarrays". International Journal of Oncology 27, no. 2 (2005): 371-376. https://doi.org/10.3892/ijo.27.2.371