Molecular factors of 5-fluorouracil metabolism in colorectal cancer: Analysis of primary tumor and lymph node metastasis

  • Authors:
    • D. Vallböhmer
    • H. Kuramochi
    • D. Shimizu
    • K. D. Danenberg
    • J. Lindebjerg
    • J. N. Nielsen
    • A. Jakobsen
    • P. V. Danenberg
  • View Affiliations

  • Published online on: February 1, 2006     https://doi.org/10.3892/ijo.28.2.527
  • Pages: 527-533
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD) and thymidine phosphorylase (TP) are predictive markers for tumor response to 5-fluorouracil-based therapies. To determine whether gene expression values measured in primary cancer tissue would be useful for prediction of response of lymph node metastases, the expressions of these genes were quantitatively analyzed in 35 pairs of primary colorectal cancer (CRC) and corresponding lymph node metastases using real-time PCR. DPD and TP mRNA levels were significantly lower in the primary colorectal tumor and lymph node metastases compared with the normal adjacent stroma tissue (p<0.01), whereas TS mRNA levels were significantly higher in the primary tumor and lymph node metastases than in the normal adjacent tissue (p<0.001). Median gene expression levels of TP and TS did not differ significantly between primary colorectal tumor and corresponding lymph node metastasis but median DPD gene expression levels in the lymph node metastases were significantly higher compared to matched primary colorectal tumors (p=0.015). There was a significant correlation for DPD, TP and TS gene expression levels between primary colorectal tumor specimens and the matched lymph node metastasis. These results suggest that biopsies of the tumor of origin may be valid for determining predictive markers for chemotherapy response in patients with metastatic CRC.

Related Articles

Journal Cover

February 2006
Volume 28 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Vallböhmer D, Kuramochi H, Shimizu D, Danenberg KD, Lindebjerg J, Nielsen JN, Jakobsen A and Danenberg PV: Molecular factors of 5-fluorouracil metabolism in colorectal cancer: Analysis of primary tumor and lymph node metastasis. Int J Oncol 28: 527-533, 2006
APA
Vallböhmer, D., Kuramochi, H., Shimizu, D., Danenberg, K.D., Lindebjerg, J., Nielsen, J.N. ... Danenberg, P.V. (2006). Molecular factors of 5-fluorouracil metabolism in colorectal cancer: Analysis of primary tumor and lymph node metastasis. International Journal of Oncology, 28, 527-533. https://doi.org/10.3892/ijo.28.2.527
MLA
Vallböhmer, D., Kuramochi, H., Shimizu, D., Danenberg, K. D., Lindebjerg, J., Nielsen, J. N., Jakobsen, A., Danenberg, P. V."Molecular factors of 5-fluorouracil metabolism in colorectal cancer: Analysis of primary tumor and lymph node metastasis". International Journal of Oncology 28.2 (2006): 527-533.
Chicago
Vallböhmer, D., Kuramochi, H., Shimizu, D., Danenberg, K. D., Lindebjerg, J., Nielsen, J. N., Jakobsen, A., Danenberg, P. V."Molecular factors of 5-fluorouracil metabolism in colorectal cancer: Analysis of primary tumor and lymph node metastasis". International Journal of Oncology 28, no. 2 (2006): 527-533. https://doi.org/10.3892/ijo.28.2.527