Anti-angiogenic action of plasma hyaluronan binding protein in human umbilical vein endothelial cells

  • Authors:
    • Ji Won Jeon
    • Hyun Seok Song
    • Eun-Joung Moon
    • Shi-Young Park
    • Myung Jin Son
    • Seung Youn Jung
    • Ji Tae Kim
    • Do-Hyun Nam
    • Nam-Ho Choi-Miura
    • Kyu-Won Kim
    • Yung-Jin Kim
  • View Affiliations

  • Published online on: July 1, 2006     https://doi.org/10.3892/ijo.29.1.209
  • Pages: 209-215
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Abstract

The kringle domain is a triple loop structure present in angiostatin and endostatin. The disulfide bond-linked kringle architectures have been known to be essential for anti-angiogenic activity. Plasma hyaluronan binding protein (PHBP) is a novel serine protease which consists of three epidermal growth factor (EGF) domains, a kringle domain, and a serine protease domain. PHBP can be cleaved autocatalytically to generate activity and is highly expressed in the human blood and liver. To determine the anti-angiogenic activities of PHBP, we purified recombinant mouse PHBP from stable cell line overexpressing PHBP and used protein in vivo and in vitro angiogenesis assays. We found that recombinant PHBP inhibits not only angiogenesis in vivo in chorioallantoic membrane (CAM) assay but also the basic fibroblast growth factor (bFGF)-induced proliferation, invasion and tube formation of human umbilical vein endothelial cells (HUVECs) in a dose-dependant manner. Moreover, we found that the kringle domain of PHBP was essential for the anti-angiogenic action of PHBP by the deletion mutants. These findings unravel a new function of PHBP as an inhibitor of the proangiogenic phenotype of vascular endothelial cells and demonstrate that the kringle domain of PHBP might be a potent novel inhibitor of activated endothelial cells in vitro and in vivo.

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July 2006
Volume 29 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Jeon JW, Song HS, Moon E, Park S, Son MJ, Jung SY, Kim JT, Nam D, Choi-Miura N, Kim K, Kim K, et al: Anti-angiogenic action of plasma hyaluronan binding protein in human umbilical vein endothelial cells. Int J Oncol 29: 209-215, 2006.
APA
Jeon, J.W., Song, H.S., Moon, E., Park, S., Son, M.J., Jung, S.Y. ... Kim, Y. (2006). Anti-angiogenic action of plasma hyaluronan binding protein in human umbilical vein endothelial cells. International Journal of Oncology, 29, 209-215. https://doi.org/10.3892/ijo.29.1.209
MLA
Jeon, J. W., Song, H. S., Moon, E., Park, S., Son, M. J., Jung, S. Y., Kim, J. T., Nam, D., Choi-Miura, N., Kim, K., Kim, Y."Anti-angiogenic action of plasma hyaluronan binding protein in human umbilical vein endothelial cells". International Journal of Oncology 29.1 (2006): 209-215.
Chicago
Jeon, J. W., Song, H. S., Moon, E., Park, S., Son, M. J., Jung, S. Y., Kim, J. T., Nam, D., Choi-Miura, N., Kim, K., Kim, Y."Anti-angiogenic action of plasma hyaluronan binding protein in human umbilical vein endothelial cells". International Journal of Oncology 29, no. 1 (2006): 209-215. https://doi.org/10.3892/ijo.29.1.209