Identification of a novel gene HEPT3 that is overexpressed in human hepatocellular carcinoma and may function through its noncoding RNA

  • Authors:
    • Mei Chung Moh
    • Lay Hoon Lee
    • Xiaodong Yang
    • Shali Shen
  • View Affiliations

  • Published online on: August 1, 2007     https://doi.org/10.3892/ijo.31.2.293
  • Pages: 293-301
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Abstract

Genetic alterations have been defined as the hallmark of cancers as they are responsible for the differences between normal and malignant phenotypes. A widely accepted approach to study genetic instability is to identify cancer-related genes, in particular, the two major groups of growth regulatory genes - oncogenes and tumour suppressor genes. Using the technique of suppression subtractive hybridisation, we identified a novel gene transcript, designated as HEPT3. RT-PCR demonstrated that HEPT3 was overexpressed in 87% (20/23) of HCC patients and in 4/5 HCC cell lines tested. Sequence analyses performed on the full-length cDNA revealed that HEPT3 is an intronless gene mapped to human chromosome 6q13-14. The gene transcript lacks an extensive open reading frame and contains an Alu sequence near the 5' terminus, indicating that HEPT3 encodes a noncoding RNA. Antisense studies on the HCC cell line HepG2 showed that, when HEPT3 expression level was reduced, cell proliferation rate was inhibited by ≈5-fold and cell colony formation was reduced by at least 50%. Our data suggest that the novel gene HEPT3 may function through its noncoding RNA and its overexpression may play a role in hepatocarcinogenesis.

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August 2007
Volume 31 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Moh MC, Lee LH, Yang X and Shen S: Identification of a novel gene HEPT3 that is overexpressed in human hepatocellular carcinoma and may function through its noncoding RNA. Int J Oncol 31: 293-301, 2007
APA
Moh, M.C., Lee, L.H., Yang, X., & Shen, S. (2007). Identification of a novel gene HEPT3 that is overexpressed in human hepatocellular carcinoma and may function through its noncoding RNA. International Journal of Oncology, 31, 293-301. https://doi.org/10.3892/ijo.31.2.293
MLA
Moh, M. C., Lee, L. H., Yang, X., Shen, S."Identification of a novel gene HEPT3 that is overexpressed in human hepatocellular carcinoma and may function through its noncoding RNA". International Journal of Oncology 31.2 (2007): 293-301.
Chicago
Moh, M. C., Lee, L. H., Yang, X., Shen, S."Identification of a novel gene HEPT3 that is overexpressed in human hepatocellular carcinoma and may function through its noncoding RNA". International Journal of Oncology 31, no. 2 (2007): 293-301. https://doi.org/10.3892/ijo.31.2.293