Mitotic catastrophe induced in HeLa cells by photodynamic treatment with Zn(II)-phthalocyanine

  • Authors:
    • Santiago Rello-Varona
    • Juan Carlos Stockert
    • Magdalena Cañete
    • Pilar Acedo
    • Ángeles Villanueva
  • View Affiliations

  • Published online on: June 1, 2008     https://doi.org/10.3892/ijo.32.6.1189
  • Pages: 1189-1196
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Abstract

Photodynamic therapy (PDT) is a tool against neoplastic and non-neoplastic diseases. PDT is capable to induce different cell death mechanisms in vitro, triggered in a dose-dependent manner. Relationships between PDT and apoptosis or necrosis induction are well-known, but other cell death mechanisms triggered after PDT are less understood. Here we present our results in p53-deficient human cervix carcinoma HeLa cells subjected to sublethal PDT treatments (mortality about 40%) using Zn(II)-phthalocyanine (ZnPc) incorporated into liposomes. We obtained a rapid metaphase blockage of cells that also showed clearly altered configurations of the mitotic spindle. Cell cycle arrest was followed by aneuploidisation and cell death with apoptotic morphology. Apoptosis was also confirmed by occurrence of PARP cleavage and Bax translocation to mitochondria. These features are components of the cell death mechanism known as mitotic catastrophe and represent, to our knowledge, the first description of this cell death modality after PDT with ZnPc.

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June 2008
Volume 32 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Rello-Varona S, Stockert JC, Cañete M, Acedo P and Villanueva Á: Mitotic catastrophe induced in HeLa cells by photodynamic treatment with Zn(II)-phthalocyanine. Int J Oncol 32: 1189-1196, 2008.
APA
Rello-Varona, S., Stockert, J.C., Cañete, M., Acedo, P., & Villanueva, Á. (2008). Mitotic catastrophe induced in HeLa cells by photodynamic treatment with Zn(II)-phthalocyanine. International Journal of Oncology, 32, 1189-1196. https://doi.org/10.3892/ijo.32.6.1189
MLA
Rello-Varona, S., Stockert, J. C., Cañete, M., Acedo, P., Villanueva, Á."Mitotic catastrophe induced in HeLa cells by photodynamic treatment with Zn(II)-phthalocyanine". International Journal of Oncology 32.6 (2008): 1189-1196.
Chicago
Rello-Varona, S., Stockert, J. C., Cañete, M., Acedo, P., Villanueva, Á."Mitotic catastrophe induced in HeLa cells by photodynamic treatment with Zn(II)-phthalocyanine". International Journal of Oncology 32, no. 6 (2008): 1189-1196. https://doi.org/10.3892/ijo.32.6.1189