Profiling of transcripts and proteins modulated by K-ras oncogene in the lung tissues of K-ras transgenic mice by omics approaches

  • Authors:
    • Sojung Lee
    • Jungwoo Kang
    • Minchul Cho
    • Eunhee Seo
    • Heesook Choi
    • Eunjin Kim
    • Junghee Kim
    • Heejong Kim
    • Gum Yong Kang
    • Kwang Pyo Kim
    • Young-Ho Park
    • Dae-Yeul Yu
    • Young Na Yum
    • Sue-Nie Park
    • Do-Young Yoon
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/ijo_00000138
  • Pages: 161-172
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The mutated K-ras gene is involved in ≈30% of human cancers. In order to search for K-ras oncogene-induced modulators in lung tissues of K-ras transgenic mice, we performed microarray and proteomics (LC/ESI-MS/MS) analysis. Genes (RAB27b RAS family, IL-1RA, IL-33, chemokine ligand 6, epiregulin, EGF-like domain and cathepsin) related to cancer development (Wnt signaling pathway) and inflammation (chemokine/cytokine signaling pathway, Toll receptor signaling) were up-regulated while genes (troponin, tropomodulin 2, endothelial lipase, FGFR4, integrin α8 and adenylate cyclase 8) related to the tumor suppression such as p53 pathway, TGF-β signaling pathway and cadherin signaling pathway were down-regulated by K-ras oncogene. Proteomics approach revealed that up-regulated proteins in lung adenomas of K-ras mice were classified as follows: proteins related to the metabolism/catabolism (increased from 7 to 22% by K-ras gene), proteins related to translation/transcription and nucleotide (from 4 to 6%), proteins related to signal transduction (from 3 to 5%), proteins related to phosphorylation (from 1 to 2%). ATP synthase, Ras oncogene family, cytochrome c oxidase, flavoprotein, TEF 1, adipoprotein A-1 BP, glutathione oxidase, fatty acid BP 4, diaphorase 1, MAPK4 and transgelin were up-regulated by K-ras oncogene. However, integrin α1, Ras-interacting protein (Rain), endothelin-converting enzyme-1d and splicing factor 3b were down-regulated. These studies suggest that genes related to cancer development and inflammation were up-regulated while genes related to the tumor suppression were down-regulated by K-ras, resulting in the tumor growth. Putative biomarkers such as cell cycle related genes (Cdc37), cancer cell adhesion (Glycam 1, integrin α8, integrin αX and Clec4n), signal transduction (Tlr2, IL-33, and Ccbp2), migration (Ccr1, Ccl6, and diaphorase 1 (Cyb5r3) and cancer development (epiregulin) can be useful for diagnosis and as prognosis markers and some of the target molecules can be applied for prevention of cancer.

Related Articles

Journal Cover

January 2009
Volume 34 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lee S, Kang J, Cho M, Seo E, Choi H, Kim E, Kim J, Kim H, Kang GY, Kim KP, Kim KP, et al: Profiling of transcripts and proteins modulated by K-ras oncogene in the lung tissues of K-ras transgenic mice by omics approaches. Int J Oncol 34: 161-172, 2009.
APA
Lee, S., Kang, J., Cho, M., Seo, E., Choi, H., Kim, E. ... Yoon, D. (2009). Profiling of transcripts and proteins modulated by K-ras oncogene in the lung tissues of K-ras transgenic mice by omics approaches. International Journal of Oncology, 34, 161-172. https://doi.org/10.3892/ijo_00000138
MLA
Lee, S., Kang, J., Cho, M., Seo, E., Choi, H., Kim, E., Kim, J., Kim, H., Kang, G. Y., Kim, K. P., Park, Y., Yu, D., Yum, Y. N., Park, S., Yoon, D."Profiling of transcripts and proteins modulated by K-ras oncogene in the lung tissues of K-ras transgenic mice by omics approaches". International Journal of Oncology 34.1 (2009): 161-172.
Chicago
Lee, S., Kang, J., Cho, M., Seo, E., Choi, H., Kim, E., Kim, J., Kim, H., Kang, G. Y., Kim, K. P., Park, Y., Yu, D., Yum, Y. N., Park, S., Yoon, D."Profiling of transcripts and proteins modulated by K-ras oncogene in the lung tissues of K-ras transgenic mice by omics approaches". International Journal of Oncology 34, no. 1 (2009): 161-172. https://doi.org/10.3892/ijo_00000138