PKCε induces Bcl-2 by activating CREB

  • Authors:
    • Eswar Shankar
    • Soumya Krishnamurthy
    • Rajiv Paranandi
    • Alakananda Basu
  • View Affiliations

  • Published online on: April 1, 2010     https://doi.org/10.3892/ijo_00000566
  • Pages: 883-888
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Abstract

Protein kinase C epsilon (PKCε) is a transforming oncogene and an important anti-apoptotic protein. We previously demonstrated that overexpression of PKCε in MCF-7 breast cancer cells caused an increase in anti-apoptotic Bcl-2 and a decrease in pro-apoptotic Bid, attenuating tumor necrosis factor-α (TNF)-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis. The objective of our present study was to determine the mode of induction of Bcl-2 by PKCε in breast cancer cells. siRNA silencing of either PKCε or Akt in MCF-7 cells, which overexpress Akt, decreased Bcl-2 protein and mRNA levels. However, knockdown of PKCε, but not Akt, led to the decrease in Bcl-2 at both protein and mRNA levels in MDA-MB-231 breast cancer cells, which overexpress PKCε but contain little constitutively-active Akt. Knockdown of PKCε decreased phosphorylation of cAMP response element-binding protein (CREB) at Ser133 in MDA-MB-231 cells, and depletion of CREB by siRNA decreased Bcl-2 at both the protein and mRNA levels. In addition, knockdown of CREB sensitized MDA-MB-231 cells to TRAIL-mediated cell death. These results suggest that PKCε regulates Bcl-2 induction through activation of the transcription factor CREB.

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April 2010
Volume 36 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Shankar E, Krishnamurthy S, Paranandi R and Basu A: PKCε induces Bcl-2 by activating CREB. Int J Oncol 36: 883-888, 2010
APA
Shankar, E., Krishnamurthy, S., Paranandi, R., & Basu, A. (2010). PKCε induces Bcl-2 by activating CREB. International Journal of Oncology, 36, 883-888. https://doi.org/10.3892/ijo_00000566
MLA
Shankar, E., Krishnamurthy, S., Paranandi, R., Basu, A."PKCε induces Bcl-2 by activating CREB". International Journal of Oncology 36.4 (2010): 883-888.
Chicago
Shankar, E., Krishnamurthy, S., Paranandi, R., Basu, A."PKCε induces Bcl-2 by activating CREB". International Journal of Oncology 36, no. 4 (2010): 883-888. https://doi.org/10.3892/ijo_00000566