Resistance to chemotherapeutic agents and promotion of transforming activity mediated by embryonic stem cell-expressed Ras (ERas) signal in neuroblastoma cells

  • Authors:
    • Mineyoshi Aoyama
    • Hiromi Kataoka
    • Eiji Kubota
    • Toyohiro Tada
    • Kiyofumi Asai
  • View Affiliations

  • Published online on: October 1, 2010     https://doi.org/10.3892/ijo_00000752
  • Pages: 1011-1016
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Abstract

Neuroblastoma is a common childhood tumor derived from neural crest precursor cells. In the present study, we investigated the expression and function of embryonic stem cell-expressed Ras (ERas), a novel Ras family protein previously reported as the specific expression gene in embryonic stem cells (ES cells), in neuroblastoma cell lines. Our results showed that the expressions of ERas were detected in neuroblastoma cell lines by RT-PCR and Western blotting. Therefore, we transfected a full length ERas expression vector into the neuroblastoma cell line SH-SY5Y, which has weak endogenous expression of ERas, and obtained clones with higher levels of expression. Overexpression of ERas did not increase the growth rate of the ERas transfectants but promoted their transforming activity. The ERas transfectants were more resistant to all the chemotherapy agents than the parental cell line. The ability of ERas to rescue cells from the toxic effect of chemotherapeutic agents was inhibited by the phosphatidylinositol 3'-kinase (PI3K) inhibitor PD294002. These results show that the ERas/PI3K pathway may provide resistance to chemotherapy and promote transforming activity in neuroblastoma.

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October 2010
Volume 37 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Aoyama M, Kataoka H, Kubota E, Tada T and Asai K: Resistance to chemotherapeutic agents and promotion of transforming activity mediated by embryonic stem cell-expressed Ras (ERas) signal in neuroblastoma cells. Int J Oncol 37: 1011-1016, 2010.
APA
Aoyama, M., Kataoka, H., Kubota, E., Tada, T., & Asai, K. (2010). Resistance to chemotherapeutic agents and promotion of transforming activity mediated by embryonic stem cell-expressed Ras (ERas) signal in neuroblastoma cells. International Journal of Oncology, 37, 1011-1016. https://doi.org/10.3892/ijo_00000752
MLA
Aoyama, M., Kataoka, H., Kubota, E., Tada, T., Asai, K."Resistance to chemotherapeutic agents and promotion of transforming activity mediated by embryonic stem cell-expressed Ras (ERas) signal in neuroblastoma cells". International Journal of Oncology 37.4 (2010): 1011-1016.
Chicago
Aoyama, M., Kataoka, H., Kubota, E., Tada, T., Asai, K."Resistance to chemotherapeutic agents and promotion of transforming activity mediated by embryonic stem cell-expressed Ras (ERas) signal in neuroblastoma cells". International Journal of Oncology 37, no. 4 (2010): 1011-1016. https://doi.org/10.3892/ijo_00000752