Open Access

Critical involvement of RQCD1 in the EGFR-Akt pathway in mammary carcinogenesis

  • Authors:
    • Masahiko Ajiro
    • Toshihiko Nishidate
    • Toyomasa Katagiri
    • Yusuke Nakamura
  • View Affiliations

  • Published online on: November 1, 2010     https://doi.org/10.3892/ijo_00000760
  • Pages: 1085-1093
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Abstract

We previously reported an important role of RQCD1 in mammary carcinogenesis through the interaction with Grb10 interacting GYF protein 1 (GIGYF1), Grb10 interacting GYF protein 2 (GIGYF2) and growth factor receptor binding protein 10 (Grb10). In this study, we investigated the biological mechanism of RQCD1 in regulation of the Akt activity as the downstream signal of epidermal growth factor receptor (EGFR). Knockdown of RQCD1 reduced the Akt phosphorylation level that was induced by epidermal growth factor (EGF) stimulation. We found a possible formation of the big complex involved in the Akt activity including Akt, EGFR, GIGYF1 and GIGYF2, Grb10 and RQCD1. We subsequently defined that a region corresponding to 620-665th amino acids of GIGYF1 and 667-712th amino acids of GIGYF2 interacted with RQCD1. Furthermore, we found that RQCD1 was required for enhancement of the interaction of Grb10 with GIGYF1 and GIGYF2. Our findings in this study imply the functional mechanism of RQCD1 in the Akt activity regulation as a mediator in the EGFR-signaling pathway.

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November 2010
Volume 37 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Ajiro, M., Nishidate, T., Katagiri, T., & Nakamura, Y. (2010). Critical involvement of RQCD1 in the EGFR-Akt pathway in mammary carcinogenesis. International Journal of Oncology, 37, 1085-1093. https://doi.org/10.3892/ijo_00000760
MLA
Ajiro, M., Nishidate, T., Katagiri, T., Nakamura, Y."Critical involvement of RQCD1 in the EGFR-Akt pathway in mammary carcinogenesis". International Journal of Oncology 37.5 (2010): 1085-1093.
Chicago
Ajiro, M., Nishidate, T., Katagiri, T., Nakamura, Y."Critical involvement of RQCD1 in the EGFR-Akt pathway in mammary carcinogenesis". International Journal of Oncology 37, no. 5 (2010): 1085-1093. https://doi.org/10.3892/ijo_00000760