Cancer stem-like cells of glioblastoma characteristically express MMP-13 and display highly invasive activity

  • Authors:
    • Akihiro Inoue
    • Hisaaki Takahashi
    • Hironobu Harada
    • Shohei Kohno
    • Shiro Ohue
    • Kana Kobayashi
    • Hajime Yano
    • Junya Tanaka
    • Takanori Ohnishi
  • View Affiliations

  • Published online on: November 1, 2010     https://doi.org/10.3892/ijo_00000764
  • Pages: 1121-1131
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Abstract

Glioblastoma is the most malignant type of primary brain tumor that has been shown to contain a small population of cancer stem cells. Recent studies have suggested that cancer stem cells cause tumor recurrence based on their resistance to radiotherapy and chemotherapy. Although the highly invasive nature of glioblastoma cells is also implicated in the failure of current therapies, it is not clear whether cancer stem cells are involved in invasiveness. In this study, we isolated tumor sphere-forming cells bearing cancer stem-like characteristics such as self-renewal, multipotency, drug-resistibility, and in vivo tumorigenicity, from the human glioblastoma cell line U251, under serum-free neural stem cell culture condition, and assessed their migratory and invasive ability. These cells showed enhanced migratory and invasive ability on both Matrigel and organotypic brain slices compared to parental U251 cells. The expression of matrix metalloproteinase (MMP)-13 was specifically expressed in tumor sphere-forming cells derived from U251 and primary human glioma cells. Knockdown of MMP-13 expression by shRNA suppressed the migration and invasion of these cells. The results suggest that the highly invasive potential of cancer stem cells depends on MMP-13 enzymatic activity, thus MMP-13 might be a potential therapeutic target for glioblastomas.

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November 2010
Volume 37 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Inoue A, Takahashi H, Harada H, Kohno S, Ohue S, Kobayashi K, Yano H, Tanaka J and Ohnishi T: Cancer stem-like cells of glioblastoma characteristically express MMP-13 and display highly invasive activity. Int J Oncol 37: 1121-1131, 2010
APA
Inoue, A., Takahashi, H., Harada, H., Kohno, S., Ohue, S., Kobayashi, K. ... Ohnishi, T. (2010). Cancer stem-like cells of glioblastoma characteristically express MMP-13 and display highly invasive activity. International Journal of Oncology, 37, 1121-1131. https://doi.org/10.3892/ijo_00000764
MLA
Inoue, A., Takahashi, H., Harada, H., Kohno, S., Ohue, S., Kobayashi, K., Yano, H., Tanaka, J., Ohnishi, T."Cancer stem-like cells of glioblastoma characteristically express MMP-13 and display highly invasive activity". International Journal of Oncology 37.5 (2010): 1121-1131.
Chicago
Inoue, A., Takahashi, H., Harada, H., Kohno, S., Ohue, S., Kobayashi, K., Yano, H., Tanaka, J., Ohnishi, T."Cancer stem-like cells of glioblastoma characteristically express MMP-13 and display highly invasive activity". International Journal of Oncology 37, no. 5 (2010): 1121-1131. https://doi.org/10.3892/ijo_00000764