Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53

  • Authors:
    • Shiho Ohnishi
    • Manabu Futamura
    • Hiroki Kamino
    • Yasuyuki Nakamura
    • Noriaki Kitamura
    • Yuji Miyamoto
    • Takafumi Miyamoto
    • Daisuke Shinogi
    • Olga Goda
    • Hirofumi Arakawa
  • View Affiliations

  • Published online on: November 1, 2010     https://doi.org/10.3892/ijo_00000765
  • Pages: 1133-1141
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Abstract

NEEP21, also designated D4S234E or NSG1, is an endosomal protein expressed in neuronal cells under normal conditions. Here, we report that NEEP21 is a direct transcriptional target gene of the tumor suppressor p53. NEEP21 expression is inducible in non-neuronal human cancer cell lines by exposure to adriamycin, hydrogen peroxide, UV and γ-ray in a p53-dependent manner. Chromatin immunoprecipitation assay indicated that a potential p53-binding site (p53BS) is located in intron 1 of the NEEP21 gene. A reporter assay confirmed that p53BS has p53-responsive activity. The heterologous luciferase gene containing p53BS is also transactivated by p73-β and p63-γ. The introduction of the NEEP21 gene into various cancer cell lines suppressed cell growth. Infection with an adenovirus vector containing NEEP21 induced apoptotic cell death via caspase-3 activation in many cancer cell lines. The expression of NEEP21 mRNA was remarkably induced by γ-ray irradiation in the spleen of p53+/+ mice but not in that of p53−/− mice. These results suggest that NEEP21 may play a critical role in apoptosis as a mediator of p53.

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November 2010
Volume 37 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ohnishi S, Futamura M, Kamino H, Nakamura Y, Kitamura N, Miyamoto Y, Miyamoto T, Shinogi D, Goda O, Arakawa H, Arakawa H, et al: Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53. Int J Oncol 37: 1133-1141, 2010
APA
Ohnishi, S., Futamura, M., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, Y. ... Arakawa, H. (2010). Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53. International Journal of Oncology, 37, 1133-1141. https://doi.org/10.3892/ijo_00000765
MLA
Ohnishi, S., Futamura, M., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, Y., Miyamoto, T., Shinogi, D., Goda, O., Arakawa, H."Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53". International Journal of Oncology 37.5 (2010): 1133-1141.
Chicago
Ohnishi, S., Futamura, M., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, Y., Miyamoto, T., Shinogi, D., Goda, O., Arakawa, H."Identification of NEEP21, encoding neuron-enriched endosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53". International Journal of Oncology 37, no. 5 (2010): 1133-1141. https://doi.org/10.3892/ijo_00000765