Zinc oxide nanoparticles induce photocatalytic cell death in human head and neck squamous cell carcinoma cell lines in vitro

  • Authors:
    • Stephan Hackenberg
    • Agmal Scherzed
    • Michael Kessler
    • Katrin Froelich
    • Christian Ginzkey
    • Christian Koehler
    • Marc Burghartz
    • Rudolf Hagen
    • Norbert Kleinsasser
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijo_00000812
  • Pages: 1583-1590
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Abstract

The aim of this study was to determine the photocatalytic effects of zinc oxide (ZnO) NPs in combination with UVA-1 in human head and neck squamous cell carcinoma (HNSCC) cell lines in vitro. NP characteristics and intracellular distribution were described by transmission electron microscopy (TEM). After pre-incubation with ZnO NPs in concentrations of 0.002-20 µg/ml, the HNSCC cell lines HLaC 78 and UD-SCC 7A as well as primary oral mucosa cells (pOMCs) were treated with UVA-1. Cell survival and vitality was observed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide-(MTT)-assay and fluorescein diacetate test. Apoptosis was assessed by annexin-V propidium iodide flow cytometry. Intranuclear distribution of the rod-shaped particles was observed in 3.5% of HNSCC and in 0.5% of pOMCs. UVA-1 irradiation of 15 min in combination with 0.2 and 2 µg/ml of ZnO NP dispersion was shown to reduce the vitality of cancer cell lines significantly in comparison to cells without NP exposure or UVA-1 treatment only. For HLaC 78, a significant reduction in viable cells was already seen at 10 min of UVA-1 treatment and a ZnO NP concentration of 2 µg/ml. Flow cytometry indicated that cell death occurred primarily through necrosis. In pOMCs, vitality was not influenced either by UVA-1 treatment or ZnO NP exposure up to 2 µg/ml or a combination of both. ZnO NPs showed cytotoxicity at 20 µg/ml without UVA-1. Due to their photocatalytic properties, ZnO NPs may induce cell death in human HNSCC cell lines in vitro. Further studies will evaluate a possible benefit in adjuvant cancer therapy.

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December 2010
Volume 37 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hackenberg S, Scherzed A, Kessler M, Froelich K, Ginzkey C, Koehler C, Burghartz M, Hagen R and Kleinsasser N: Zinc oxide nanoparticles induce photocatalytic cell death in human head and neck squamous cell carcinoma cell lines in vitro. Int J Oncol 37: 1583-1590, 2010
APA
Hackenberg, S., Scherzed, A., Kessler, M., Froelich, K., Ginzkey, C., Koehler, C. ... Kleinsasser, N. (2010). Zinc oxide nanoparticles induce photocatalytic cell death in human head and neck squamous cell carcinoma cell lines in vitro. International Journal of Oncology, 37, 1583-1590. https://doi.org/10.3892/ijo_00000812
MLA
Hackenberg, S., Scherzed, A., Kessler, M., Froelich, K., Ginzkey, C., Koehler, C., Burghartz, M., Hagen, R., Kleinsasser, N."Zinc oxide nanoparticles induce photocatalytic cell death in human head and neck squamous cell carcinoma cell lines in vitro". International Journal of Oncology 37.6 (2010): 1583-1590.
Chicago
Hackenberg, S., Scherzed, A., Kessler, M., Froelich, K., Ginzkey, C., Koehler, C., Burghartz, M., Hagen, R., Kleinsasser, N."Zinc oxide nanoparticles induce photocatalytic cell death in human head and neck squamous cell carcinoma cell lines in vitro". International Journal of Oncology 37, no. 6 (2010): 1583-1590. https://doi.org/10.3892/ijo_00000812