Circulating melanoma cells and survival in metastatic melanoma

  • Authors:
    • C. Rao
    • T. Bui
    • M. Connelly
    • G. Doyle
    • I. Karydis
    • M. R. Middleton
    • G. Clack
    • M. Malone
    • F. A.W. Coumans
    • L.W. M.M. Terstappen
  • View Affiliations

  • Published online on: January 3, 2011     https://doi.org/10.3892/ijo.2011.896
  • Pages: 755-760
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Abstract

A validated assay for the enumeration of circulating melanoma cells (CMCs) may facilitate the development of more effective therapies for metastatic melanoma patients. In this study CD146+ cells were immunomagnetically enriched from 7.5 ml of blood. Isolated cells were fluorescently stained with DAPI, anti-molecular weight melanoma-associated antigen (HMW-MAA), anti-CD45 and CD34 and Ki67. CMCs were identified as CD146+, HMW-MAA+, CD45-, CD34-, Ki67-/+ cells. Eighty-eight percent of spiked SK-MEL28 cells in 7.5 ml blood were recovered. In all 55 healthy donors ≤1 CMCs were detected in 7.5 ml of blood. A retrospective analysis was conducted comparing CMC counts and overall survival in 79 blood samples from 44 melanoma patients. CMCs ranged from 0 to 8,042 per 7.5 ml. Two or more CMCs were detected in 18 (23%) of the patients and 30-100% (mean 84%) of the CMCs expressed the proliferation marker Ki67. Patients with ≥2 CMCs per 7.5 ml of whole blood, as compared with the group with <2 CMCs, had a shorter overall survival (2.0 months vs. 12.1 months, P=0.001).

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March 2011
Volume 38 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Rao C, Bui T, Connelly M, Doyle G, Karydis I, Middleton MR, Clack G, Malone M, Coumans FA, Terstappen LM, Terstappen LM, et al: Circulating melanoma cells and survival in metastatic melanoma. Int J Oncol 38: 755-760, 2011
APA
Rao, C., Bui, T., Connelly, M., Doyle, G., Karydis, I., Middleton, M.R. ... Terstappen, L.M. (2011). Circulating melanoma cells and survival in metastatic melanoma. International Journal of Oncology, 38, 755-760. https://doi.org/10.3892/ijo.2011.896
MLA
Rao, C., Bui, T., Connelly, M., Doyle, G., Karydis, I., Middleton, M. R., Clack, G., Malone, M., Coumans, F. A., Terstappen, L. M."Circulating melanoma cells and survival in metastatic melanoma". International Journal of Oncology 38.3 (2011): 755-760.
Chicago
Rao, C., Bui, T., Connelly, M., Doyle, G., Karydis, I., Middleton, M. R., Clack, G., Malone, M., Coumans, F. A., Terstappen, L. M."Circulating melanoma cells and survival in metastatic melanoma". International Journal of Oncology 38, no. 3 (2011): 755-760. https://doi.org/10.3892/ijo.2011.896