Tripchlorolide induces cell death in lung cancer cells by autophagy

  • Authors:
    • Limin Chen
    • Qing Liu
    • Zhenghui Huang
    • Feng Wu
    • Zhiying Li
    • Xiangqi Chen
    • Tingyan Lin
  • View Affiliations

  • Published online on: December 1, 2011     https://doi.org/10.3892/ijo.2011.1278
  • Pages: 1066-1070
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Abstract

It has been demonstrated that triptolide inhibits the growth of several types of cancer cells in vitro and prevents tumor growth in vivo by inducing apoptosis and autophagy. Here we showed that Tripchlorolide (T4) significantly suppressed the proliferation of A549 cells in a dose- and time-dependent manner. This suppressive effect was diminished when cells were pretreated with 3-Methylamphetamine (3-MA). After the cells were treated with T4, the LC3 II protein expression was significantly increased, and autophagosomes were observed by TEM. However, almost no apoptosis was observed in A549 treated with T4. These results suggest that T4 induces A549 cell death predominantly through the activation of the autophagy pathway instead of the apoptosis pathway.
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April 2012
Volume 40 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Chen L, Liu Q, Huang Z, Wu F, Li Z, Chen X and Lin T: Tripchlorolide induces cell death in lung cancer cells by autophagy . Int J Oncol 40: 1066-1070, 2012
APA
Chen, L., Liu, Q., Huang, Z., Wu, F., Li, Z., Chen, X., & Lin, T. (2012). Tripchlorolide induces cell death in lung cancer cells by autophagy . International Journal of Oncology, 40, 1066-1070. https://doi.org/10.3892/ijo.2011.1278
MLA
Chen, L., Liu, Q., Huang, Z., Wu, F., Li, Z., Chen, X., Lin, T."Tripchlorolide induces cell death in lung cancer cells by autophagy ". International Journal of Oncology 40.4 (2012): 1066-1070.
Chicago
Chen, L., Liu, Q., Huang, Z., Wu, F., Li, Z., Chen, X., Lin, T."Tripchlorolide induces cell death in lung cancer cells by autophagy ". International Journal of Oncology 40, no. 4 (2012): 1066-1070. https://doi.org/10.3892/ijo.2011.1278