Homeobox gene expression profile indicates HOXA5 as a candidate prognostic marker in oral squamous cell carcinoma

  • Authors:
    • Camila Oliveira Rodini
    • Flávia Caló Aquino Xavier
    • Katiúcia Batista Silva Paiva
    • Maria Fernanda De Souza Setúbal Destro
    • Raquel Ajub Moyses
    • Pedro Michaluarte
    • Marcos Brasilino Carvalho
    • Erica Erina Fukuyama
    • Head and Neck Genome Project Gencapo
    • Eloiza Helena Tajara
    • Oswaldo Keith Okamoto
    • Fabio Daumas Nunes
  • View Affiliations

  • Published online on: December 29, 2011     https://doi.org/10.3892/ijo.2011.1321
  • Pages: 1180-1188
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Abstract

The search for molecular markers to improve diagnosis, individualize treatment and predict behavior of tumors has been the focus of several studies. This study aimed to analyze homeobox gene expression profile in oral squamous cell carcinoma (OSCC) as well as to investigate whether some of these genes are relevant molecular markers of prognosis and/or tumor aggressiveness. Homeobox gene expression levels were assessed by microarrays and qRT-PCR in OSCC tissues and adjacent non-cancerous matched tissues (margin), as well as in OSCC cell lines. Analysis of microarray data revealed the expression of 147 homeobox genes, including one set of six at least 2-fold up-regulated, and another set of 34 at least 2-fold down-regulated homeobox genes in OSCC. After qRT-PCR assays, the three most up-regulated homeobox genes (HOXA5, HOXD10 and HOXD11) revealed higher and statistically significant expression levels in OSCC samples when compared to margins. Patients presenting lower expression of HOXA5 had poorer prognosis compared to those with higher expression (P=0.03). Additionally, the status of HOXA5, HOXD10 and HOXD11 expression levels in OSCC cell lines also showed a significant up-regulation when compared to normal oral keratinocytes. Results confirm the presence of three significantly upregulated (>4-fold) homeobox genes (HOXA5, HOXD10 and HOXD11) in OSCC that may play a significant role in the pathogenesis of these tumors. Moreover, since lower levels of HOXA5 predict poor prognosis, this gene may be a novel candidate for development of therapeutic strategies in OSCC.
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April 2012
Volume 40 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Rodini CO, Xavier FC, Paiva KB, De Souza Setúbal Destro MF, Moyses RA, Michaluarte P, Carvalho MB, Fukuyama EE, Gencapo H, Tajara EH, Tajara EH, et al: Homeobox gene expression profile indicates HOXA5 as a candidate prognostic marker in oral squamous cell carcinoma. Int J Oncol 40: 1180-1188, 2012
APA
Rodini, C.O., Xavier, F.C., Paiva, K.B., De Souza Setúbal Destro, M.F., Moyses, R.A., Michaluarte, P. ... Nunes, F.D. (2012). Homeobox gene expression profile indicates HOXA5 as a candidate prognostic marker in oral squamous cell carcinoma. International Journal of Oncology, 40, 1180-1188. https://doi.org/10.3892/ijo.2011.1321
MLA
Rodini, C. O., Xavier, F. C., Paiva, K. B., De Souza Setúbal Destro, M. F., Moyses, R. A., Michaluarte, P., Carvalho, M. B., Fukuyama, E. E., Gencapo, H., Tajara, E. H., Okamoto, O. K., Nunes, F. D."Homeobox gene expression profile indicates HOXA5 as a candidate prognostic marker in oral squamous cell carcinoma". International Journal of Oncology 40.4 (2012): 1180-1188.
Chicago
Rodini, C. O., Xavier, F. C., Paiva, K. B., De Souza Setúbal Destro, M. F., Moyses, R. A., Michaluarte, P., Carvalho, M. B., Fukuyama, E. E., Gencapo, H., Tajara, E. H., Okamoto, O. K., Nunes, F. D."Homeobox gene expression profile indicates HOXA5 as a candidate prognostic marker in oral squamous cell carcinoma". International Journal of Oncology 40, no. 4 (2012): 1180-1188. https://doi.org/10.3892/ijo.2011.1321