Lewis Y regulates signaling molecules of the transforming growth factor β pathway in ovarian carcinoma-derived RMG-I cells

  • Authors:
    • Fei-Fei Li
    • Juan-Juan Liu
    • Da-Wo Liu
    • Bei Lin
    • Ying-Ying Hao
    • Jian-Ping Cong
    • Lian-Cheng Zhu
    • Song Gao
    • Shu-Lan Zhang
    • Masao Iwamori
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  • Published online on: December 13, 2011     https://doi.org/10.3892/ijo.2011.1296
  • Pages: 1196-1202
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Abstract

LeY (Lewis Y) is a difucosylated oligosaccharide carried by glycoconjugates on the cell surface. Elevation of LeY is frequently observed in epithelial-derived cancers and is correlated to pathological staging and prognosis. To study the role of LeY on cancer cells, a stably LeY-overexpressing cell line, RMG-I-H, was developed previously by transfection of the α1,2-fucosyltransferase gene, a key enzyme that catalyzes the synthesis of LeY, into ovarian carcinoma-derived RMG-I cells. Our studies have shown that LeY is involved in the changes in biological behavior of RMG-I-H cells. However, the mechanism is still largely unknown. In this study, we determined the structural relationship and co-localization between LeY and TβRI/TβRII, respectively, and the potential cellular signaling mechanism was also investigated. We found that both TβRI and TβRII contain the LeY structure, and the level of LeY in TβRI and TβRII in RMG-I-H cells was significantly increased. Overexpression of LeY up-regulates the phosphorylation of ERK, Akt and down-regulates the phosphorylation of Smad2/3. In addition, the phosphorylation intensity was attenuated significantly by LeY monoantibody. These findings suggest that LeY is involved in the changes in biological behavior through TGF‑β receptors via Smad, ERK/MAPK and PI3K/Akt signaling pathways. We suggest that LeY may be an important composition of growth factor receptors and could be an attractive candidate for cancer diagnosis and treatment.
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April 2012
Volume 40 Issue 4

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Spandidos Publications style
Li F, Liu J, Liu D, Lin B, Hao Y, Cong J, Zhu L, Gao S, Zhang S, Iwamori M, Iwamori M, et al: Lewis Y regulates signaling molecules of the transforming growth factor β pathway in ovarian carcinoma-derived RMG-I cells. Int J Oncol 40: 1196-1202, 2012
APA
Li, F., Liu, J., Liu, D., Lin, B., Hao, Y., Cong, J. ... Iwamori, M. (2012). Lewis Y regulates signaling molecules of the transforming growth factor β pathway in ovarian carcinoma-derived RMG-I cells. International Journal of Oncology, 40, 1196-1202. https://doi.org/10.3892/ijo.2011.1296
MLA
Li, F., Liu, J., Liu, D., Lin, B., Hao, Y., Cong, J., Zhu, L., Gao, S., Zhang, S., Iwamori, M."Lewis Y regulates signaling molecules of the transforming growth factor β pathway in ovarian carcinoma-derived RMG-I cells". International Journal of Oncology 40.4 (2012): 1196-1202.
Chicago
Li, F., Liu, J., Liu, D., Lin, B., Hao, Y., Cong, J., Zhu, L., Gao, S., Zhang, S., Iwamori, M."Lewis Y regulates signaling molecules of the transforming growth factor β pathway in ovarian carcinoma-derived RMG-I cells". International Journal of Oncology 40, no. 4 (2012): 1196-1202. https://doi.org/10.3892/ijo.2011.1296